通过聚合酶链反应检测耐阿米卡星不动杆菌属中的aac(6')-I基因。
Detection of aac(6')-I genes in amikacin-resistant Acinetobacter spp. by PCR.
作者信息
Ploy M C, Giamarellou H, Bourlioux P, Courvalin P, Lambert T
机构信息
Unité des Agents Antibactériens, Institut Pasteur, Centre National de la Recherche Scientifique, Paris, France.
出版信息
Antimicrob Agents Chemother. 1994 Dec;38(12):2925-8. doi: 10.1128/AAC.38.12.2925.
The distribution of aac(6')-I genes in 62 strains of Acinetobacter spp. resistant to amikacin, netilmicin, and tobramycin and susceptible to gentamicin, a phenotype compatible with synthesis of an AAC(6')-I enzyme, was studied by PCR and by DNA hybridization. Both methods gave similar results. Among the 51 Acinetobacter baumannii strains, aac(6')-Ib was found in 19 isolates and aac(6')-Ih was found in the remaining strains. The aac(6')-Ig gene was present in all 10 A. haemolyticus strains studied and was detected only in this species. A pair of degenerate oligonucleotides complementary to conserved regions of aac(6')-Ic, -Id, -If, -Ig, and -Ih enabled detection of these genes and also of aac(6')-Ij, recently recognized in Acinetobacter sp. strain 13.
采用聚合酶链反应（PCR）和DNA杂交技术，研究了62株对阿米卡星、奈替米星和妥布霉素耐药但对庆大霉素敏感的不动杆菌属菌株中aac(6')-I基因的分布情况，这种表型与AAC(6')-I酶的合成相符。两种方法得出了相似的结果。在51株鲍曼不动杆菌中，19株分离菌中发现了aac(6')-Ib，其余菌株中发现了aac(6')-Ih。在所研究的全部10株溶血不动杆菌中均存在aac(6')-Ig基因，且仅在该菌种中检测到。一对与aac(6')-Ic、-Id、-If、-Ig和-Ih保守区域互补的简并寡核苷酸，能够检测到这些基因以及最近在不动杆菌属13号菌株中发现的aac(6')-Ij。
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