乳糖操纵子DNA片段的核磁共振氢谱研究。

1H NMR studies of lac-operator DNA fragments.

作者信息

Zuiderweg E R, Scheek R M, Veeneman G, van Boom J H, Kaptein R, Rüterjans H, Beyreuther K

出版信息

Nucleic Acids Res. 1981 Dec 11;9(23):6553-69. doi: 10.1093/nar/9.23.6553.

DOI:10.1093/nar/9.23.6553

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327622/

Abstract

The hydrogen-bonded imino protons of a 14 base pair double-stranded DNA fragment comprising one half of the lac operator of E. coli were investigated by 360 MHz H NMR. From combined melting studies of this synthetic 14 b.p. fragment and its two constituent 7 b.p. fragments a nearly complete assignment for the low-field proton resonances was obtained. The experimental spectra are compared with calculated spectra and with the spectrum of a 51 b.p. DNA restriction fragment from E. coli containing the complete lac operator. Structural information on these oligonucleotides is presented. This study is a prerequisite for future 1H NMR investigations of the interaction of the lac operator with the lac repressor.

摘要

利用360兆赫的氢核磁共振对包含大肠杆菌乳糖操纵子一半的14个碱基对双链DNA片段中通过氢键连接的亚氨基质子进行了研究。通过对这个合成的14碱基对片段及其两个组成的7碱基对片段进行联合熔解研究，获得了低场质子共振的几乎完整的归属。将实验光谱与计算光谱以及来自大肠杆菌的包含完整乳糖操纵子的51碱基对DNA限制片段的光谱进行了比较。给出了这些寡核苷酸的结构信息。这项研究是未来对乳糖操纵子与乳糖阻遏物相互作用进行氢核磁共振研究的前提条件。

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Proc Natl Acad Sci U S A. 1981 Apr;78(4):2179-83. doi: 10.1073/pnas.78.4.2179.

3

A 300- and 600-MHz proton nuclear magnetic resonance investigation of a 12 base pair deoxyribonucleic acid restriction fragment: relaxation behavior of the low-field resonances in water.对一个12碱基对脱氧核糖核酸限制性片段进行的300兆赫和600兆赫质子核磁共振研究：水中低场共振的弛豫行为

Biochemistry. 1981 Jun 23;20(13):3756-64. doi: 10.1021/bi00516a014.

4

Optimised parameters for A-DNA and B-DNA.A-DNA和B-DNA的优化参数。

Biochem Biophys Res Commun. 1972 Jun 28;47(6):1504-9. doi: 10.1016/0006-291X(72)90243-4.

5

The molecular mechanism of thermal unfolding of Escherichia coli formylmethionine transfer RNA.大肠杆菌甲酰甲硫氨酸转移核糖核酸热解折叠的分子机制。

J Mol Biol. 1974 Jul 25;87(1):63-88. doi: 10.1016/0022-2836(74)90560-9.

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