构建缺失锰和铁超氧化物歧化酶基因的大肠杆菌K-12菌株及其在克隆嗜肺军团菌铁超氧化物歧化酶基因中的应用。
Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila.
作者信息
Steinman H M
机构信息
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Mol Gen Genet. 1992 Apr;232(3):427-30. doi: 10.1007/BF00266247.
PMID:1588912
Abstract
An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.
摘要
构建了一株缺失超氧化物歧化酶A（SodA）和超氧化物歧化酶B（SodB）（锰和铁超氧化物歧化酶）的大肠杆菌K-12菌株，并通过Southern印迹法、酶活性测定和生理学分析对其进行了表征。利用该超氧化物歧化酶缺失菌株，通过互补对百草枯抗性来克隆嗜肺军团菌的铁超氧化物歧化酶基因。
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