大肠杆菌K12 nusB（groNB）基因的核苷酸序列。
The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.
作者信息
Swindle J, Ajioka J, Dawson D, Myers R, Carroll D, Georgopoulos C
出版信息
Nucleic Acids Res. 1984 Jun 25;12(12):4977-85. doi: 10.1093/nar/12.12.4977.
Abstract
The nusB (groNB) gene product of Escherichia coli plays a pivotal role in allowing bacteriophage lambda N protein to function as an antiterminator of mRNA transcription and in modulating host gene expression. In addition it is essential for bacterial viability since mutations in it result in a cold-sensitivity phenotype for growth. We have previously cloned the nusB gene and shown it to code for a 14,500-Mr protein. Here we present the primary DNA sequence of the nusB gene. From the sequence we deduce that it codes for a slightly basic protein (21 basic as opposed to 20 acidic amino acids) composed of 139 amino acids with a cumulative 15,689-Mr. The predicted N-terminal amino acid sequence as well as the overall amino acid composition agrees well with that of the purified protein.
摘要
大肠杆菌的nusB（groNB）基因产物在使噬菌体λ N蛋白发挥mRNA转录抗终止子功能以及调节宿主基因表达方面起着关键作用。此外，它对细菌的生存能力至关重要，因为其中的突变会导致生长出现冷敏表型。我们之前已克隆出nusB基因，并表明它编码一种14500道尔顿的蛋白质。在此，我们展示nusB基因的初级DNA序列。从该序列我们推断，它编码一种略呈碱性的蛋白质（21个碱性氨基酸，相对20个酸性氨基酸），由139个氨基酸组成，累积分子量为15689道尔顿。预测的N端氨基酸序列以及整体氨基酸组成与纯化蛋白的情况非常吻合。
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