噬菌体P2阻遏基因和操纵区的DNA序列。
DNA sequences of the repressor gene and operator region of bacteriophage P2.
作者信息
Ljungquist E, Kockum K, Bertani L E
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3988-92. doi: 10.1073/pnas.81.13.3988.
Abstract
The nucleotide sequence of the repressor gene C of the temperate phage P2 has been determined. It codes for a nonbasic polypeptide, 99 amino acids long. Twelve repressor-defective mutants have been mapped. All but one are located within the presumed coding part of the gene. There is a strong promoter sequence and an 8-base-pair inverted repeat preceding the gene. The P2 repressor protein shows structural similarity to other DNA-binding proteins. The operator region for the early replication functions was located by sequencing the DNA of three virulent mutants. The sequence indicates that there are two repressor-binding sites. In addition, one of the sites shows sequence homology with part of the operator region of the biotin operon of Escherichia coli.
摘要
温和噬菌体P2的阻遏基因C的核苷酸序列已被确定。它编码一种非碱性多肽，长度为99个氨基酸。已对12个阻遏缺陷型突变体进行了定位。除一个之外，其余所有突变体都位于该基因的推测编码区内。该基因之前有一个强启动子序列和一个8碱基对的反向重复序列。P2阻遏蛋白与其他DNA结合蛋白在结构上具有相似性。通过对三个烈性突变体的DNA进行测序，确定了早期复制功能的操纵区。序列表明存在两个阻遏蛋白结合位点。此外，其中一个位点与大肠杆菌生物素操纵子的操纵区部分具有序列同源性。
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