Adlercreutz H, Partanen P, Virkola P, Liewendahl K, Turunen M J
Scand J Clin Lab Invest. 1984 Oct;44(6):519-28. doi: 10.1080/00365518409083605.
Five guaiac-based faecal occult blood tests (Fecatest, Fecatwin Sensitive, Fecatwin, Haemoccult, Hemafecia) were compared in vitro and in vivo and the effect of time between sample application and development of the colour reaction was studied. In both type of experiments the sensitivity of Fecatest, Fecatwin Sensitive and Fecatwin was higher when the colour reaction was developed at 24-72 h than at 2 h after application of the sample but for Haemoccult and Hemafecia the sensitivity in in vivo experiments was constant or tended to decrease with time. In upper gastrointestinal bleeding false negative test results were obtained for Fecatest, Fecatwin Sensitive, Fecatwin, Haemoccult and Hemafecia in 21, 23, 38, 43 and 43%, respectively. In colorectal bleeding the corresponding percentages were 10, 10, 35, 35 and 35. Using the 51Cr-erythrocyte method, quantitative estimation of intestinal bleeding was performed in 20 patients with verified colorectal cancer and in 11 control patients. When the patient was bleeding from left hemicolon 29% of the Fecatwin, Haemoccult and Hemafecia test results were negative, 10% were negative for Fecatest and 14% for Fecatwin Sensitive. In patients with tumours in right hemicolon Fecatest and Fecatwin Sensitive were positive in all samples, whereas the other tests were negative in 10%. Experiments revealed that the proportion of false positives due to dietary factors correlated with the sensitivity of the tests.
对五种基于愈创木脂的粪便潜血试验（Fecatest、Fecatwin Sensitive、Fecatwin、Haemoccult、Hemafecia）进行了体外和体内比较，并研究了样本施加与显色反应之间的时间影响。在这两种类型的实验中，当在样本施加后24 - 72小时进行显色反应时，Fecatest、Fecatwin Sensitive和Fecatwin的灵敏度高于2小时时，但对于Haemoccult和Hemafecia，体内实验中的灵敏度保持恒定或随时间趋于下降。在上消化道出血中，Fecatest、Fecatwin Sensitive、Fecatwin、Haemoccult和Hemafecia的假阴性检测结果分别为21%、23%、38%、43%和43%。在结肠直肠出血中，相应的百分比分别为10%、10%、35%、35%和35%。使用51Cr - 红细胞法，对20例经证实患有结肠直肠癌的患者和11例对照患者进行了肠道出血的定量估计。当患者左半结肠出血时，Fecatwin、Haemoccult和Hemafecia检测结果的29%为阴性，Fecatest为10%，Fecatwin Sensitive为14%。在右半结肠有肿瘤的患者中，Fecatest和Fecatwin Sensitive在所有样本中均为阳性，而其他检测有10%为阴性。实验表明，饮食因素导致的假阳性比例与检测的灵敏度相关。
