Lysikiewicz A, Enhorning G
Am J Obstet Gynecol. 1983 Jan 15;145(2):229-33. doi: 10.1016/0002-9378(83)90497-0.
A rapid and accurate technique for assessment of spermatozoa motility has been developed. With the use of tricolor photography with sequential exposures in green, red, and blue light, spermatozoa were photographed in Makler chambers with a depth of 10 mu. Exposure in green light had a 1-second duration and was immediately followed by the red light from an electronic flash, and 0.05 second later by the blue light from a second flash. With this system, spermatozoa with motility would leave a green track, at the end of which they would appear first red and then blue. The colors and their intensity were chosen so that, when superimposed on nonmotile spermatozoa, the latter would appear white. By projection of the transparent film onto white paper, spermatozoa, motile and nonmotile, were easily counted and the percentage and motility was determined. Also, concentration could be calculated because the photograph represented a semen volume of 0.001 mu. Measurement of the green track and conversion to true length in micrometers gave the velocity in micrometers per second.
已开发出一种快速准确的评估精子活力的技术。通过使用在绿光、红光和蓝光下进行连续曝光的三色摄影，在深度为10微米的Makler计数板中对精子进行拍照。绿光曝光持续1秒，随后立即是电子闪光灯发出的红光，0.05秒后是第二个闪光灯发出的蓝光。使用该系统，有活力的精子会留下绿色轨迹，在轨迹末端它们会先呈现红色然后是蓝色。选择这些颜色及其强度是为了使它们叠加在无活力精子上时，后者会呈现白色。通过将透明胶片投影到白纸上，可以轻松对有活力和无活力的精子进行计数，并确定活力百分比。此外，由于照片代表的精液体积为0.001微升，因此可以计算精子浓度。测量绿色轨迹并将其转换为以微米为单位的真实长度，可得出以微米每秒为单位的速度。
