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1
Bacteriophage MS2 lysis protein does not require coat protein to mediate cell lysis.噬菌体MS2裂解蛋白介导细胞裂解不需要衣壳蛋白。
J Bacteriol. 1983 Feb;153(2):1098-100. doi: 10.1128/jb.153.2.1098-1100.1983.
2
Leakage induced in Escherichia coli cells by A protein-RNA complexes from bacteriophage f2.噬菌体f2的A蛋白-RNA复合物在大肠杆菌细胞中诱导的渗漏。
J Virol. 1981 Jul;39(1):60-6. doi: 10.1128/JVI.39.1.60-66.1981.
3
Lysis induction of Escherichia coli by the cloned lysis protein of the phage MS2 depends on the presence of osmoregulatory membrane-derived oligosaccharides.噬菌体MS2的克隆裂解蛋白对大肠杆菌的裂解诱导取决于渗透调节性膜衍生寡糖的存在。
J Biol Chem. 1988 Mar 15;263(8):3539-41.
4
Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium.用于定量裂解大肠杆菌和鼠伤寒沙门氏菌的简便温和方法。
J Bacteriol. 1984 Apr;158(1):354-6. doi: 10.1128/jb.158.1.354-356.1984.
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The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein.RNA噬菌体Qβ的裂解功能由成熟(A2)蛋白介导。
EMBO J. 1983;2(9):1521-6. doi: 10.1002/j.1460-2075.1983.tb01617.x.
6
Analysis of six new genes encoding lysis proteins and coat proteins in Escherichia coli group A RNA phages.对大肠杆菌A组RNA噬菌体中六个编码裂解蛋白和衣壳蛋白的新基因的分析。
Microbiol Immunol. 2006;50(1):61-6. doi: 10.1111/j.1348-0421.2006.tb03772.x.
7
MS2 Lysis of Escherichia coli Depends on Host Chaperone DnaJ.大肠杆菌的MS2裂解依赖于宿主伴侣蛋白DnaJ。
J Bacteriol. 2017 May 25;199(12). doi: 10.1128/JB.00058-17. Print 2017 Jun 15.
8
Analysis of phage MS2 coat protein mutants expressed from a reconstituted phagemid reveals that proline 78 is essential for viral infectivity.对从重组噬菌粒表达的噬菌体MS2外壳蛋白突变体的分析表明,脯氨酸78对病毒感染性至关重要。
J Mol Biol. 1997 Feb 14;266(1):1-7. doi: 10.1006/jmbi.1996.0786.
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Novel "Superspreader" Bacteriophages Promote Horizontal Gene Transfer by Transformation.新型“超级传播者”噬菌体通过转化促进水平基因转移。
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Escherichia coli traD(Ts) mutant temperature sensitive for assembly of RNA bacteriophage MS2.大肠杆菌traD(Ts)突变体对RNA噬菌体MS2的组装具有温度敏感性。
J Virol. 1981 Jun;38(3):833-9. doi: 10.1128/JVI.38.3.833-839.1981.

引用本文的文献

1
Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.噬菌体单基因裂解:在细菌细胞壁上寻找弱点。
J Biol Chem. 2019 Mar 8;294(10):3350-3358. doi: 10.1074/jbc.TM118.001773. Epub 2018 Nov 12.
2
ydfD encodes a novel lytic protein in Escherichia coli.ydfD在大肠杆菌中编码一种新型裂解蛋白。
FEMS Microbiol Lett. 2016 Mar;363(6). doi: 10.1093/femsle/fnw039. Epub 2016 Feb 16.
3
Genetic analysis of MraY inhibition by the phiX174 protein E.噬菌体φX174蛋白E对MraY抑制作用的遗传分析
Genetics. 2008 Nov;180(3):1459-66. doi: 10.1534/genetics.108.093443. Epub 2008 Sep 14.
4
Expression of a gene in a 400-base-pair fragment of colicin plasmid ColE2-P9 is sufficient to cause host cell lysis.大肠杆菌素质粒ColE2-P9的一个400个碱基对片段中的基因表达足以导致宿主细胞裂解。
J Bacteriol. 1983 Oct;156(1):109-14. doi: 10.1128/jb.156.1.109-114.1983.
5
The amino terminal half of the MS2-coded lysis protein is dispensable for function: implications for our understanding of coding region overlaps.MS2编码的裂解蛋白的氨基末端一半对于功能而言是可有可无的:这对我们理解编码区重叠具有启示意义。
EMBO J. 1985 Dec 1;4(12):3315-20. doi: 10.1002/j.1460-2075.1985.tb04082.x.
6
Induction of the autolytic system of Escherichia coli by specific insertion of bacteriophage MS2 lysis protein into the bacterial cell envelope.通过将噬菌体MS2裂解蛋白特异性插入细菌细胞膜来诱导大肠杆菌自溶系统。
J Bacteriol. 1988 Nov;170(11):5027-33. doi: 10.1128/jb.170.11.5027-5033.1988.
7
Differential induction of Escherichia coli autolysis by penicillin and the bacteriophage phi X174 gene E product.青霉素和噬菌体φX174基因E产物对大肠杆菌自溶的差异诱导作用。
J Bacteriol. 1986 May;166(2):683-5. doi: 10.1128/jb.166.2.683-685.1986.
8
Specific localization of the lysis protein of bacteriophage MS2 in membrane adhesion sites of Escherichia coli.噬菌体MS2裂解蛋白在大肠杆菌膜黏附位点的特异性定位
J Bacteriol. 1989 Jun;171(6):3331-6. doi: 10.1128/jb.171.6.3331-3336.1989.
9
Bacteriophage lysis: mechanism and regulation.噬菌体裂解:机制与调控。
Microbiol Rev. 1992 Sep;56(3):430-81. doi: 10.1128/mr.56.3.430-481.1992.
10
A bifunctional vector system for controlled expression and subsequent release of the cloned gene product by phi X174 lysis protein-E.
Appl Microbiol Biotechnol. 1990 Aug;33(5):564-8. doi: 10.1007/BF00172552.

本文引用的文献

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Functional expression of individual plasmid-coded RNA bacteriophage MS2 genes.单个质粒编码的 RNA 噬菌体 MS2 基因的功能表达。
EMBO J. 1982;1(2):205-9. doi: 10.1002/j.1460-2075.1982.tb01148.x.
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Lysis gene expression of RNA phage MS2 depends on a frameshift during translation of the overlapping coat protein gene.RNA噬菌体MS2的裂解基因表达取决于重叠衣壳蛋白基因翻译过程中的移码。
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Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.信号肽氨基末端区域的正电荷在蛋白质跨膜分泌中的作用。
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Targets of penicillin action in Escherichia coli.青霉素在大肠杆菌中的作用靶点。
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Nucleotide sequence at the binding site for coat protein on RNA of bacteriophage R17.噬菌体R17 RNA上外壳蛋白结合位点的核苷酸序列。
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Studies on the bacteriophages MS2. XXXIII. Comparison of the nucleotide sequences in related bacteriophage RNAs.噬菌体MS2的研究。三十三。相关噬菌体RNA核苷酸序列的比较。
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Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function.哺乳动物核糖体与MS2噬菌体RNA的结合揭示了一个编码裂解功能的重叠基因。
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Characterization of Op3, a lysis-defective mutant of bacteriophage f2.噬菌体f2的裂解缺陷型突变体Op3的特性分析。
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Normal tRNAs promote ribosomal frameshifting.正常的转运RNA促进核糖体移码。
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噬菌体MS2裂解蛋白介导细胞裂解不需要衣壳蛋白。

Bacteriophage MS2 lysis protein does not require coat protein to mediate cell lysis.

作者信息

Coleman J, Inouye M, Atkins J

出版信息

J Bacteriol. 1983 Feb;153(2):1098-100. doi: 10.1128/jb.153.2.1098-1100.1983.

DOI:10.1128/jb.153.2.1098-1100.1983
PMID:6337118
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC221743/
Abstract

We inserted all but the extreme 5' end of a DNA copy of the bacteriophage MS2 lysis gene downstream of a lac-induced promoter on a multicopy plasmid. Upon induction, cells harboring this plasmid began to lyse, showing that phage coat protein is not required for the lytic process itself.

摘要

我们将噬菌体MS2裂解基因DNA拷贝的除最末端5'端之外的所有部分,插入到多拷贝质粒上乳糖诱导型启动子下游。诱导后,携带该质粒的细胞开始裂解,表明裂解过程本身并不需要噬菌体外壳蛋白。