Coleman J, Inouye M, Atkins J
J Bacteriol. 1983 Feb;153(2):1098-100. doi: 10.1128/jb.153.2.1098-1100.1983.
We inserted all but the extreme 5' end of a DNA copy of the bacteriophage MS2 lysis gene downstream of a lac-induced promoter on a multicopy plasmid. Upon induction, cells harboring this plasmid began to lyse, showing that phage coat protein is not required for the lytic process itself.
我们将噬菌体MS2裂解基因DNA拷贝的除最末端5'端之外的所有部分,插入到多拷贝质粒上乳糖诱导型启动子下游。诱导后,携带该质粒的细胞开始裂解,表明裂解过程本身并不需要噬菌体外壳蛋白。