Britton P, Cármenes R S, Page K W, Garwes D J, Parral F
Division of Microbiology, A.F.R.C. Institute for Animal Disease Research, Compton Laboratory, Compton, nr. Newbury, Bertshire, RG16 ONN, UK.
Mol Microbiol. 1988 Jan;2(1):89-99. doi: 10.1111/j.1365-2958.1988.tb00010.x.
Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA which was sequenced. Two non-overlapping open reading frames (ORFs) were identified. The largest, encoding a polypeptide of 382 amino acids (relative molecular mass (M ) 43 483), was shown to be the viral nucleoprotein gene. The second ORF, found 3'to the larger ORF, encodes a polypeptide of 78 amino acids (M 9068) which has yet to be assigned to a viral product. The nucleoprotein gene was expressed in yeast cells under the control of two types of yeast promoters: the constitutive PGK promoter, and the inducible GAL1 promoter. Yeast cells containing recombinant plasmids, with the nucleoprotein gene in the correct orientation, produced a polypeptide of M, 47000, identical to the viral product, that reacted with a specific monoclonal antibody.
来自猪传染性胃肠炎病毒（TGEV）强毒株的亚基因组mRNA被用于制备cDNA并进行测序。鉴定出两个不重叠的开放阅读框（ORF）。其中最大的一个编码一个由382个氨基酸组成的多肽（相对分子质量（M）为43483），被证明是病毒核蛋白基因。第二个ORF位于较大ORF的3'端，编码一个由78个氨基酸组成的多肽（M为9068），其功能尚未确定为病毒产物。核蛋白基因在两种酵母启动子的控制下在酵母细胞中表达：组成型PGK启动子和诱导型GAL1启动子。含有重组质粒且核蛋白基因方向正确的酵母细胞产生了一个M为47000的多肽，与病毒产物相同，并且能与一种特异性单克隆抗体发生反应。
