Bedell G W, Soll D R
Infect Immun. 1979 Oct;26(1):348-54. doi: 10.1128/iai.26.1.348-354.1979.
In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc added to growth cultures grown at 25 degrees C (i) cause a twofold increase in the final concentration of spheres at sationary phase, (ii) result in an asynchronous block in the budding cycle at stationary phase, (iii) completely suppress mycelium formation in two independently isolated human strains which produce low but significant levels of mycelia at stationary phase, and (iv) completely suppress mycelium formation in cultures of mutant M10, in which over 60% of the cells form mycelia at stationary phase. In contrast, micromolar concentrations of zinc do not inhibit mycelium formation induced by releasing cells from stationary-phase cultures into fresh medium at 37 degrees C. In addition, if zinc is present in the growth medium of the initial culture at 25 degrees C, the average time of subsequent mycelium formation after release into fresh medium at 37 degrees C is halved. It is demonstrated that the above effects are specific to zinc. The possibility of alterante pathways for mycelium formation is suggested, and the medical implications of this possibility are discussed.
在本分析中，我们详细研究了低浓度锌对白色念珠菌生长和形态转变的影响。有证据表明，添加到在25℃培养的生长培养基中的微摩尔浓度的锌：（i）导致稳定期球体的最终浓度增加两倍；（ii）导致稳定期出芽周期出现异步阻滞；（iii）在两个独立分离的人类菌株中完全抑制菌丝体形成，这两个菌株在稳定期产生低但显著水平的菌丝体；（iv）在突变体M10的培养物中完全抑制菌丝体形成，在该突变体中超过60%的细胞在稳定期形成菌丝体。相比之下，微摩尔浓度的锌不会抑制将稳定期培养物中的细胞释放到37℃的新鲜培养基中所诱导的菌丝体形成。此外，如果在25℃的初始培养基中存在锌，那么在释放到37℃的新鲜培养基后随后形成菌丝体的平均时间会减半。结果表明上述影响是锌特有的。我们提出了菌丝体形成的替代途径的可能性，并讨论了这种可能性的医学意义。
