Sun C S, Wyde P R, Wilson S Z, Knight V
Am Rev Respir Dis. 1983 Apr;127(4):460-4. doi: 10.1164/arrd.1983.127.4.460.
Pneumonia induced in cotton rats (Sigmodon hispidus) after inoculation of respiratory syncytial virus (RSV) was accompanied by the appearance of leukocytes in infected lungs. The number of these leukocytes increased until Day 5 after inoculation when sixfold more leukocytes were recovered from infected lungs using transpleural lavage than were recovered from lungs of uninfected control animals. Although macrophages and lymphocytes constituted approximately 65 and 25%, respectively, of the cells observed in lavage suspensions from both infected and uninfected lungs, only leukocytes in suspensions from infected animals appeared to be activated, as judged by cellular morphologic examination, cytochemical staining, and bactericidal activity. Leukocytes from lungs and adjacent lymph nodes of infected cotton rats, but not similar cells from uninfected animals, caused significant chromium release when they were added to primary cotton rat embryo and Hep-2 tissue culture cells infected with RSV in cytotoxicity assays. Cytotoxic activity peaked 5 days after inoculation, was neither virus-specific nor H-2 restricted, and was associated with both adherent and nonadherent fractions of lung cells. There was a close temporal relationship between the appearance of cytotoxic activity in the lung and termination of virus replication in this organ, suggesting a role for cytotoxic effector cells in recovery from respiratory syncytial virus infection of cotton rats.
在棉鼠（棉鼠属）接种呼吸道合胞病毒（RSV）后诱发的肺炎，伴随着受感染肺组织中白细胞的出现。这些白细胞的数量不断增加，直至接种后第5天，此时通过经胸灌洗从受感染肺组织中回收的白细胞数量比从未受感染对照动物的肺组织中回收的白细胞数量多5倍。尽管巨噬细胞和淋巴细胞分别约占从受感染和未受感染肺组织的灌洗悬液中观察到的细胞的65%和25%，但根据细胞形态学检查、细胞化学染色和杀菌活性判断，只有来自受感染动物悬液中的白细胞似乎被激活。在细胞毒性试验中，将来自受感染棉鼠肺组织和邻近淋巴结的白细胞（而非来自未受感染动物的类似细胞）添加到感染RSV的原代棉鼠胚胎和Hep-2组织培养细胞中时，会导致显著的铬释放。细胞毒性活性在接种后5天达到峰值，既不是病毒特异性的，也不受H-2限制，并且与肺细胞的贴壁和非贴壁部分都有关。肺组织中细胞毒性活性的出现与该器官中病毒复制的终止之间存在密切的时间关系，这表明细胞毒性效应细胞在棉鼠从呼吸道合胞病毒感染中恢复过程中发挥了作用。
