Kitano N, Taminato T, Ida T, Seno M, Seino Y, Matsukura S, Kuno S, Imura H
Department of Internal Medicine, Kyoto University School of Medicine, Japan.
Diabet Med. 1988 Mar;5(2):139-44. doi: 10.1111/j.1464-5491.1988.tb00960.x.
An attempt was made to detect islet cell surface antibodies (ICSAb) using solubilized islet-cell glycoproteins as antigens. Isolated rat islets were labelled with 125I-wheat germ agglutinin (WGA) and solubilized by Nonidet P-40 with sonication. 125I-WGA-bound islet-cell proteins were incubated with test sera, and bound antibodies were precipitated with anti-human IgG or IgM immunobeads. Serum which had a bound percent beyond the mean plus 2SD of control sera was defined as antibody-positive. Results obtained by this method correlated well with those by the immunofluorescence method of detecting ICSAb. Prevalences of antibodies were 21/114 (18%) for IgG antibodies and 9/114 (8%) for IgM antibodies in patients with insulin-dependent diabetes mellitus (IDDM). The prevalence was highest for both IgG and IgM antibodies in patients within a year of the onset of disease (38 and 25%, respectively), and decreased thereafter. The prevalence of IgM antibodies was lower than that of IgG antibodies at all stages. In NIDDM patients, the prevalence of antibodies was 5/72 (7%) for both IgG and IgM antibodies. If these preliminary results are confirmed, this radioassay may be developed to detect antibodies against islet cell membrane proteins on a large scale.
尝试以溶解的胰岛细胞糖蛋白作为抗原检测胰岛细胞表面抗体（ICSAb）。将分离的大鼠胰岛用125I-小麦胚凝集素（WGA）标记，并用去氧胆酸钠（Nonidet P-40）超声处理使其溶解。将与125I-WGA结合的胰岛细胞蛋白与检测血清孵育，并用抗人IgG或IgM免疫磁珠沉淀结合的抗体。结合百分比超过对照血清平均值加2个标准差的血清被定义为抗体阳性。通过该方法获得的结果与检测ICSAb的免疫荧光法的结果相关性良好。在胰岛素依赖型糖尿病（IDDM）患者中，IgG抗体的阳性率为21/114（18%），IgM抗体的阳性率为9/114（8%）。在疾病发作一年内的患者中，IgG和IgM抗体的阳性率均最高（分别为38%和25%），此后下降。在所有阶段，IgM抗体的阳性率均低于IgG抗体。在非胰岛素依赖型糖尿病（NIDDM）患者中，IgG和IgM抗体的阳性率均为5/72（7%）。如果这些初步结果得到证实，这种放射免疫测定法可能会被开发用于大规模检测针对胰岛细胞膜蛋白的抗体。
