Saffhill R, Abbott P J
Chem Biol Interact. 1983 Apr-May;44(1-2):95-110. doi: 10.1016/0009-2797(83)90132-1.
N-Acetoxy-2-acetylaminofluorene (AcO-AAF) reacts with the alternating DNA-like polynucleotides poly(dC-dG) and poly(dA-dT) in vitro to give adducts of the guanine and adenine bases similar to those reported to be formed in DNA. A previously unobserved guanine adduct was detected in the poly(dC-dG). Using a double-labelled [U-14C-dG, 8-3H-G]-poly(dC-dG) we show that this adduct does not involve the 7- or 8-positions of the guanine. Similarly a thymine adduct of unknown structure was observed in poly(dA-dT). Modification of the polymers with AcO-AAF inhibits their capacity to act as templates for Escherichia coli DNA polymerase I and mammalian DNA polymerase alpha although the binding of the polymerases to the polynucleotides is unaffected. Such modification also leads to an increase in the levels of non-complementary nucleotides incorporated into newly synthesised DNA.
N-乙酰氧基-2-乙酰氨基芴（AcO-AAF）在体外与交替的类DNA多核苷酸聚（dC-dG）和聚（dA-dT）反应，生成鸟嘌呤和腺嘌呤碱基的加合物，类似于据报道在DNA中形成的加合物。在聚（dC-dG）中检测到一种以前未观察到的鸟嘌呤加合物。使用双标记的[U-14C-dG，8-3H-G]-聚（dC-dG），我们表明这种加合物不涉及鸟嘌呤的7位或8位。类似地，在聚（dA-dT）中观察到一种结构未知的胸腺嘧啶加合物。用AcO-AAF对聚合物进行修饰会抑制它们作为大肠杆菌DNA聚合酶I和哺乳动物DNA聚合酶α模板的能力，尽管聚合酶与多核苷酸的结合不受影响。这种修饰还导致掺入新合成DNA中的非互补核苷酸水平增加。
