N-2-乙酰氨基芴与鸟嘌呤残基结合诱导聚（dG-dC）·聚（dG-dC）形成Z构象。

Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.

作者信息

Santella R M, Grunberger D, Weinstein I B, Rich A

出版信息

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1451-5. doi: 10.1073/pnas.78.3.1451.

DOI:10.1073/pnas.78.3.1451

PMID:6262801

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319148/

Abstract

Poly(dG-dC).poly(dG-dC) and poly(dG).poly(dC) were modified by treatment with N-acetoxy-N-2-acetylaminofluorene, and their conformations were examined by circular dichroism and susceptibility to nuclease S1 digestion. A sample of poly(dG-dC).poly(dG-dC) modified to an extent of 28% with acetylaminofluorene (AAF) at the C(8) position of the deoxyguanosine residues showed a circular dichroism spectrum that had the characteristics of the Z conformation seen in unmodified poly(dG-dC).poly(dG-dC) at high ethanol or salt concentrations. A sample of poly(dG-dC).poly(dG-dC) modified only 3% by AAF showed a spectrum characteristic of the B form of DNA. However, it was converted to the Z form at ethanol concentrations lower than required to convert unmodified poly(dG-dC).poly(dG-dC) from the B to the Z form. Poly(dG).poly(dC), which does not undergo the B-to-Z transition at high ethanol concentrations, did not show any large conformational changes with high AAF modification. Susceptibility to digestion with nuclease S1 also suggested differences in the conformations of the two modified polynucleotides. Poly(dG-dC).poly(dG-dC) modified by AAF to an extent of 28% was almost completely resistant to nuclease S1 digestion. However, both poly(dG).poly(dC) and DNA modified to similar levels by AAF were highly susceptible to nuclease S1 digestion. Two different conformations for AAF-modified deoxyguanosine are proposed, depending on whether its position is in alternating purine-pyrimidine sequences or in random-sequence DNA.

摘要

用N-乙酰氧基-N-2-乙酰氨基芴处理聚（dG-dC）·聚（dG-dC）和聚（dG）·聚（dC），并通过圆二色性和对核酸酶S1消化的敏感性来检测它们的构象。在脱氧鸟苷残基的C(8)位置用乙酰氨基芴（AAF）修饰至28%的聚（dG-dC）·聚（dG-dC）样品，其圆二色光谱具有在高乙醇或盐浓度下未修饰的聚（dG-dC）·聚（dG-dC）中所见的Z构象特征。仅用AAF修饰3%的聚（dG-dC）·聚（dG-dC）样品显示出DNA B型的光谱特征。然而，在乙醇浓度低于将未修饰的聚（dG-dC）·聚（dG-dC）从B型转变为Z型所需浓度时，它就转变为Z型。在高乙醇浓度下不发生B到Z转变的聚（dG）·聚（dC），在高AAF修饰时未显示出任何大的构象变化。对核酸酶S1消化的敏感性也表明两种修饰的多核苷酸构象存在差异。用AAF修饰至28%的聚（dG-dC）·聚（dG-dC）几乎完全抵抗核酸酶S1消化。然而，聚（dG）·聚（dC）和用AAF修饰至相似水平的DNA都对核酸酶S1消化高度敏感。根据AAF修饰的脱氧鸟苷的位置是在交替的嘌呤-嘧啶序列中还是在随机序列DNA中，提出了两种不同的构象。

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