N-2-乙酰氨基芴与鸟嘌呤残基结合诱导聚（dG-dC）·聚（dG-dC）形成Z构象。

Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.

作者信息

Santella R M, Grunberger D, Weinstein I B, Rich A

出版信息

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1451-5. doi: 10.1073/pnas.78.3.1451.

DOI:10.1073/pnas.78.3.1451

PMID:6262801

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319148/

Abstract

Poly(dG-dC).poly(dG-dC) and poly(dG).poly(dC) were modified by treatment with N-acetoxy-N-2-acetylaminofluorene, and their conformations were examined by circular dichroism and susceptibility to nuclease S1 digestion. A sample of poly(dG-dC).poly(dG-dC) modified to an extent of 28% with acetylaminofluorene (AAF) at the C(8) position of the deoxyguanosine residues showed a circular dichroism spectrum that had the characteristics of the Z conformation seen in unmodified poly(dG-dC).poly(dG-dC) at high ethanol or salt concentrations. A sample of poly(dG-dC).poly(dG-dC) modified only 3% by AAF showed a spectrum characteristic of the B form of DNA. However, it was converted to the Z form at ethanol concentrations lower than required to convert unmodified poly(dG-dC).poly(dG-dC) from the B to the Z form. Poly(dG).poly(dC), which does not undergo the B-to-Z transition at high ethanol concentrations, did not show any large conformational changes with high AAF modification. Susceptibility to digestion with nuclease S1 also suggested differences in the conformations of the two modified polynucleotides. Poly(dG-dC).poly(dG-dC) modified by AAF to an extent of 28% was almost completely resistant to nuclease S1 digestion. However, both poly(dG).poly(dC) and DNA modified to similar levels by AAF were highly susceptible to nuclease S1 digestion. Two different conformations for AAF-modified deoxyguanosine are proposed, depending on whether its position is in alternating purine-pyrimidine sequences or in random-sequence DNA.

摘要

用N-乙酰氧基-N-2-乙酰氨基芴处理聚（dG-dC）·聚（dG-dC）和聚（dG）·聚（dC），并通过圆二色性和对核酸酶S1消化的敏感性来检测它们的构象。在脱氧鸟苷残基的C(8)位置用乙酰氨基芴（AAF）修饰至28%的聚（dG-dC）·聚（dG-dC）样品，其圆二色光谱具有在高乙醇或盐浓度下未修饰的聚（dG-dC）·聚（dG-dC）中所见的Z构象特征。仅用AAF修饰3%的聚（dG-dC）·聚（dG-dC）样品显示出DNA B型的光谱特征。然而，在乙醇浓度低于将未修饰的聚（dG-dC）·聚（dG-dC）从B型转变为Z型所需浓度时，它就转变为Z型。在高乙醇浓度下不发生B到Z转变的聚（dG）·聚（dC），在高AAF修饰时未显示出任何大的构象变化。对核酸酶S1消化的敏感性也表明两种修饰的多核苷酸构象存在差异。用AAF修饰至28%的聚（dG-dC）·聚（dG-dC）几乎完全抵抗核酸酶S1消化。然而，聚（dG）·聚（dC）和用AAF修饰至相似水平的DNA都对核酸酶S1消化高度敏感。根据AAF修饰的脱氧鸟苷的位置是在交替的嘌呤-嘧啶序列中还是在随机序列DNA中，提出了两种不同的构象。

相似文献

Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.N-2-乙酰氨基芴与鸟嘌呤残基结合诱导聚（dG-dC）·聚（dG-dC）形成Z构象。

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1451-5. doi: 10.1073/pnas.78.3.1451.

Alternative conformations of DNA modified by N-2-acetylaminofluorene.由N-2-乙酰氨基芴修饰的DNA的不同构象

J Supramol Struct Cell Biochem. 1981;17(3):231-44. doi: 10.1002/jsscb.380170305.

Induction of the base displacement or Z conformation in DNA by N-2-acetylaminofluorene modification.N-2-乙酰氨基芴修饰诱导DNA中的碱基位移或Z构象。

Environ Health Perspect. 1983 Mar;49:107-15. doi: 10.1289/ehp.8349107.

Conformational changes of poly(dG-dC) . poly(dG-dC) modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene.经致癌物N-乙酰氧基-N-乙酰-2-氨基芴修饰的聚（dG-dC）·聚（dG-dC）的构象变化

Nucleic Acids Res. 1981 Mar 11;9(5):1241-50. doi: 10.1093/nar/9.5.1241.

Z-DNA conformation of N-2-acetylaminofluorene modified poly(dG-dC).poly(dG-dC) determined by reactivity with anti cytidine antibodies and minimized potential energy calculations.通过与抗胞嘧啶抗体反应及最小化势能计算确定的 N-2-乙酰氨基芴修饰的聚（dG-dC）·聚（dG-dC）的 Z-DNA 构象

Nucleic Acids Res. 1981 Oct 24;9(20):5459-67. doi: 10.1093/nar/9.20.5459.

Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.多聚（dG-dC）·多聚（dG-dC）的构象：被致癌物N-乙酰氧基-N-乙酰-2-氨基芴和N-羟基-N-2-氨基芴修饰后的情况

Proc Natl Acad Sci U S A. 1980 Aug;77(8):4597-601. doi: 10.1073/pnas.77.8.4597.

Conformations of poly(dG-dC).poly(dG-dC) modified by the O-acetyl derivative of the carcinogen 4-hydroxyaminoquinoline 1-oxide.由致癌物4-羟基氨基喹啉1-氧化物的O-乙酰基衍生物修饰的聚（dG-dC）.聚（dG-dC）的构象

Nucleic Acids Res. 1984 Oct 25;12(20):7915-27. doi: 10.1093/nar/12.20.7915.

An immunochemical examination of acetylaminofluorene-modified poly(dG-dC) X poly(dG-dC) in the Z-conformation.对处于Z构象的乙酰氨基芴修饰的聚（dG-dC）×聚（dG-dC）进行免疫化学检测。

J Biol Chem. 1984 Jan 10;259(1):173-8.

Spectrophotometrical and immunochemical studies on the conformational changes in poly(dG-dC).poly(dG-dC) after modification by 4-hydroxyaminoquinoline 1-oxide.4-羟基氨基喹啉1-氧化物修饰后聚（dG-dC）·聚（dG-dC）构象变化的分光光度法和免疫化学研究

Biochim Biophys Acta. 1991 Aug 27;1090(1):29-37. doi: 10.1016/0167-4781(91)90033-i.

Interaction of DNA methyltransferase with aminofluorene and N-acetylaminofluorene modified poly(dC-dG).DNA甲基转移酶与氨基芴及N - 乙酰氨基芴修饰的聚（dC - dG）的相互作用

Nucleic Acids Res. 1984 Apr 11;12(7):3357-72. doi: 10.1093/nar/12.7.3357.

引用本文的文献

High-Affinity Low-Capacity and Low-Affinity High-Capacity N-Acetyl-2-Aminofluorene (AAF) Macromolecular Binding Sites Are Revealed During the Growth Cycle of Adult Rat Hepatocytes in Primary Culture.在原代培养的成年大鼠肝细胞生长周期中，揭示了高亲和力低容量和低亲和力高容量 N-乙酰-2-氨基芴（AAF）大分子结合位点。

Toxicol Sci. 2018 May 1;163(1):35-44. doi: 10.1093/toxsci/kfy007.

AM1 Study of N-2-Acetylaminofluorene bonded to Deoxyguanosine at the Minor Adduct Site.N-2-乙酰氨基芴在次要加合物位点与脱氧鸟苷结合的AM1研究。

J Biol Phys. 2004 Jun;30(2):161-9. doi: 10.1023/B:JOBP.0000035860.68226.22.

Nucleic Acids Res. 1981 Oct 24;9(20):5459-67. doi: 10.1093/nar/9.20.5459.

Binding of [(dien)PtCl] Cl to poly(dG-dC)-poly(dG-dC) facilitates the B goes to Z conformational transition.[(二乙三胺)铂氯]氯与聚(dG-dC)-聚(dG-dC)的结合促进了B型到Z型的构象转变。

Nucleic Acids Res. 1982 Jun 11;10(11):3573-88. doi: 10.1093/nar/10.11.3573.

The B goes to Z transition of poly(dG-dC) . poly(dG-dC) modified by some platinum derivatives.聚（dG-dC）·聚（dG-dC）经某些铂衍生物修饰后的B到Z转变。

Nucleic Acids Res. 1981 Nov 11;9(21):5659-69. doi: 10.1093/nar/9.21.5659.

Simultaneous stability of short alternating Z and B helices in synthetic DNA concatamers.合成DNA串联体中短交替Z螺旋和B螺旋的同时稳定性。

Nucleic Acids Res. 1982 Jun 25;10(12):3759-68. doi: 10.1093/nar/10.12.3759.

Conformational transitions of poly(dA-dC).poly(dG-dT) induced by high salt or in ethanolic solution.高盐或乙醇溶液诱导的聚（dA-dC）·聚（dG-dT）的构象转变

Nucleic Acids Res. 1982 Feb 11;10(3):1081-91. doi: 10.1093/nar/10.3.1081.

Effect of the B--Z transition in poly(dG-m5dC) . poly(dG-m5dC) on nucleosome formation.聚（dG-m5dC）·聚（dG-m5dC）中B-Z转变对核小体形成的影响。

Proc Natl Acad Sci U S A. 1982 Mar;79(6):1771-5. doi: 10.1073/pnas.79.6.1771.

Z-DNA: vacuum ultraviolet circular dichroism.Z型DNA：真空紫外圆二色性

Proc Natl Acad Sci U S A. 1981 Aug;78(8):4801-4. doi: 10.1073/pnas.78.8.4801.

7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation.聚（dG-dC）·聚（dG-dC）中的7-甲基鸟嘌呤促进Z-DNA的形成。

Proc Natl Acad Sci U S A. 1981 Aug;78(8):4777-81. doi: 10.1073/pnas.78.8.4777.

本文引用的文献

Left-handed DNA helices.左手DNA螺旋

Nature. 1980 Feb 21;283(5749):743-5. doi: 10.1038/283743a0.

Proc Natl Acad Sci U S A. 1980 Aug;77(8):4597-601. doi: 10.1073/pnas.77.8.4597.

Coding and conformational properties of oligonucleotides modified with the carcinogen N-2-acetylaminofluorene.用致癌物N-2-乙酰氨基芴修饰的寡核苷酸的编码和构象特性。

Proc Natl Acad Sci U S A. 1970 Jun;66(2):488-94. doi: 10.1073/pnas.66.2.488.

Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性

Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.

Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism.圆二色光谱揭示溶液中双链核酸的不同构象。

Biopolymers. 1973;12(1):89-110. doi: 10.1002/bip.1973.360120109.

Modification of ribonucleic acid by chemical carcinogens. VI. Effect of N-2-acetylaminofluorene modification of guanosine on the codon function of adjacent nucleosides in oligonucleotides.化学致癌物对核糖核酸的修饰。VI. N-2-乙酰氨基芴对鸟苷的修饰对寡核苷酸中相邻核苷密码子功能的影响。

J Mol Biol. 1974 Feb 5;82(4):459-68. doi: 10.1016/0022-2836(74)90241-1.

Effect of N-2-acetylaminofluorene modification on the conformation of nucleic acids.N-2-乙酰氨基芴修饰对核酸构象的影响。

Cancer Res. 1974 Feb;34(2):319-27.

Physical studies on deoxyribonucleic acid after covalent binding of a carcinogen.致癌物共价结合后脱氧核糖核酸的物理研究

Biochemistry. 1972 Jul 4;11(14):2659-66. doi: 10.1021/bi00764a017.

Chemical carcinogens as frameshift mutagens: Salmonella DNA sequence sensitive to mutagenesis by polycyclic carcinogens.作为移码诱变剂的化学致癌物：对多环致癌物诱变敏感的沙门氏菌DNA序列

Proc Natl Acad Sci U S A. 1974 May;71(5):1612-7. doi: 10.1073/pnas.71.5.1612.

A circular dichroism study of poly dG, poly dC, and poly dG:dC.聚dG、聚dC和聚dG:dC的圆二色性研究。

Biopolymers. 1974;13(10):2087-102. doi: 10.1002/bip.1974.360131011.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。