经致癌物N-乙酰氧基-N-乙酰-2-氨基芴修饰的聚（dG-dC）·聚（dG-dC）的构象变化
Conformational changes of poly(dG-dC) . poly(dG-dC) modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene.
作者信息
Sage E, Leng M
出版信息
Nucleic Acids Res. 1981 Mar 11;9(5):1241-50. doi: 10.1093/nar/9.5.1241.
Abstract
Poly (dG-dC) . poly(dG-dC) was modified by the reaction with N-acetoxy-N-acetyl-2-aminofluorene. The conformations of poly(dG-dC) . poly(dG-dC) and of poly d(G-C)AAF were studied by circular dichroism under various experimental conditions. In 95% ethanol, the two polynucleotides adopt the A-form. In 3.9 M LiCl, the transition B-form-C-form is observed with poly(dG-dC) . poly (dG-dC) but not with poly d(G-C)AAF. In 1 mM phosphate buffer, poly d(G-C)AAF behaves as a mixture of B- and Z-form, the relative percentages depending upon the amounts of modified bases. The percentage of Z-form is decreased by addition of EDTA and is increased by addition of Mg++. Spermine favors the Z-form in modified and unmodified polynucleotides. No defect in the double helix of poly d(G-C)AAF is detected by SI endonuclease.
摘要
聚（dG-dC）·聚（dG-dC）通过与N-乙酰氧基-N-乙酰基-2-氨基芴反应进行修饰。在各种实验条件下，通过圆二色性研究了聚（dG-dC）·聚（dG-dC）和聚d（G-C）AAF的构象。在95%乙醇中，这两种多核苷酸呈现A构象。在3.9 M LiCl中，聚（dG-dC）·聚（dG-dC）观察到B型向C型的转变，而聚d（G-C）AAF则未观察到。在1 mM磷酸盐缓冲液中，聚d（G-C）AAF表现为B型和Z型的混合物，相对比例取决于修饰碱基的数量。加入EDTA会降低Z型的比例，加入Mg++则会增加Z型的比例。精胺有利于修饰和未修饰多核苷酸中的Z型。SI核酸酶未检测到聚d（G-C）AAF双螺旋结构存在缺陷。
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