Bedouelle H, Hofnung M
Mol Gen Genet. 1982;185(1):82-7. doi: 10.1007/BF00333794.
The malB region in E. coli is composed of two operons, malEFG and malK-lamB, transcribed divergently from a control region located between the malE and malK genes. We have established the DNA sequence of a 600 base pair segment which contains the sites necessary for initiation of transcription of the malB operons and the translation start sites for malE and malK. We have also determined the position of a 147 base pair deletion, malB delta 100, in this sequence. The control region contains two short segments (about 35 base pairs) showing unusual distributions of bases: the GC content (85%) is higher than average and most of the purines (85%) are on one strand. We discuss the possible relevance of this and other salient features of the DNA sequence to the mechanisms by which the expression of the malB region is positively regulated.
大肠杆菌中的malB区域由两个操纵子组成，即malEFG和malK-lamB，它们从位于malE和malK基因之间的一个控制区域向相反方向转录。我们已经确定了一段600个碱基对片段的DNA序列，该片段包含启动malB操纵子转录所需的位点以及malE和malK的翻译起始位点。我们还确定了该序列中一个147个碱基对缺失（malB delta 100）的位置。控制区域包含两个短片段（约35个碱基对），其碱基分布异常：GC含量（85%）高于平均水平，且大多数嘌呤（85%）位于一条链上。我们讨论了该DNA序列的这一特征及其他显著特征与malB区域表达受到正调控的机制之间可能存在的相关性。
Zotero 插件