利用巨噬细胞进行体外评估麻风分枝杆菌活力和药物敏感性的快速放射性标记微培养方法。
Rapid, radiolabeled-microculture method that uses macrophages for in vitro evaluation of Mycobacterium leprae viability and drug susceptibility.
作者信息
Mittal A, Sathish M, Seshadri P S, Nath I
出版信息
J Clin Microbiol. 1983 Apr;17(4):704-7. doi: 10.1128/jcm.17.4.704-707.1983.
Abstract
This paper describes a microculture rapid assay using radiolabeling and mouse macrophages to determine the viability and the drug susceptibility or resistance of Mycobacterium leprae. Comparison of M. leprae resident macrophage cultures maintained in 96-well flat-bottomed plates showed results for viability and susceptibility or resistance to dapsone that were similar to results for concurrent cultures in Leighton tubes with greater numbers of bacilli and macrophages.
摘要
本文描述了一种使用放射性标记和小鼠巨噬细胞的微量培养快速测定法，以确定麻风分枝杆菌的活力以及对药物的敏感性或耐药性。对在96孔平底培养板中维持的麻风分枝杆菌驻留巨噬细胞培养物进行比较，结果显示其活力以及对氨苯砜的敏感性或耐药性与在含有更多杆菌和巨噬细胞的Leighton管中同时进行的培养物的结果相似。
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J Clin Microbiol. 1983 Apr;17(4):704-7. doi: 10.1128/jcm.17.4.704-707.1983.
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本文引用的文献
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Rapid, radiolabeled macrophage culture method for detection of dapsone-resistant Mycobacterium leprae.用于检测耐氨苯砜麻风分枝杆菌的快速放射性标记巨噬细胞培养方法。
Antimicrob Agents Chemother. 1982 Jan;21(1):26-32. doi: 10.1128/AAC.21.1.26.
2
The uptake of 3H-thymidine in Mycobacterium leprae inoculated mouse macrophage cultures as a rapid indicator of bacillary viability. Factors influencing the specificity of the in vitro assay.
Int J Lepr Other Mycobact Dis. 1981 Jun;49(2):187-93.
3
Incorporation of 3H-thymidine in Mycobacterium leprae within differentiated human macrophages.3H-胸腺嘧啶核苷在分化的人类巨噬细胞内掺入麻风分枝杆菌的情况。
J Med Microbiol. 1981 Aug;14(3):279-93. doi: 10.1099/00222615-14-3-279.
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Enhanced susceptibility of thymectomized and irradiated mice to infection with Mycobacterium leprae.胸腺切除及受辐照小鼠对麻风分枝杆菌感染的易感性增强。
Nature. 1966 Aug 6;211(5049):657-8. doi: 10.1038/211657a0.
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A method for counting acid-fast bacteria.一种抗酸菌计数方法。
Int J Lepr Other Mycobact Dis. 1968 Jan-Mar;36(1):78-82.
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