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乳过氧化物酶和碘甘氨酸催化的碘化作用标记流感嗜血杆菌的内膜和外膜蛋白。

Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae.

作者信息

Loeb M R, Smith D H

出版信息

J Bacteriol. 1983 Jul;155(1):443-6. doi: 10.1128/jb.155.1.443-446.1983.

DOI:10.1128/jb.155.1.443-446.1983
PMID:6345513
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217703/
Abstract

Both inner and outer membrane proteins of Haemophilus influenzae type b were labeled by iodination procedures believed to be specific for exposed surface proteins only. It is suggested that this is due to specific properties of the outer membrane of H. influenzae and that use of these procedures with other gram-negative bacteria be evaluated carefully.

摘要

b型流感嗜血杆菌的内膜蛋白和外膜蛋白均通过碘化程序进行标记，据信该程序仅对暴露的表面蛋白具有特异性。有人认为，这是由于流感嗜血杆菌外膜的特殊性质所致，并且应仔细评估将这些程序用于其他革兰氏阴性菌的情况。

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本文引用的文献

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Methods Enzymol. 1980;70(A):214-20. doi: 10.1016/s0076-6879(80)70051-4.
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Structural and functional evidence of cooperativity between membranes and cell wall in bacteria.
Int Rev Cytol Suppl. 1981;12:39-70. doi: 10.1016/b978-0-12-364373-5.50012-3.
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Outer membrane of Escherichia coli: properties of the F sex factor traT protein which is involved in surface exclusion.大肠杆菌外膜：参与表面排斥的F性因子traT蛋白的特性
Infect Immun. 1984 Oct;46(1):245-50. doi: 10.1128/iai.46.1.245-250.1984.
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Isolation of a major cell envelope protein from Fusobacterium nucleatum.从具核梭杆菌中分离一种主要的细胞包膜蛋白。
Infect Immun. 1984 May;44(2):386-93. doi: 10.1128/iai.44.2.386-393.1984.
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Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1.鳗弧菌的铁调节外膜蛋白OM2由毒力质粒pJM1编码。
J Bacteriol. 1985 Feb;161(2):736-42. doi: 10.1128/jb.161.2.736-742.1985.
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Structure and surface exposure of protein IIs of Neisseria gonorrhoeae JS3.淋病奈瑟菌JS3蛋白II的结构与表面暴露情况
Infect Immun. 1985 May;48(2):452-7. doi: 10.1128/iai.48.2.452-457.1985.
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Infect Immun. 1987 May;55(5):1314-20. doi: 10.1128/iai.55.5.1314-1320.1987.
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