用于酶联免疫吸附测定的抗体-酶偶联物的简易制备方法。
Simple method for preparation of antibody-enzyme conjugates for enzyme-linked immunosorbent assays.
作者信息
De Jong P J
出版信息
J Clin Microbiol. 1983 May;17(5):928-30. doi: 10.1128/jcm.17.5.928-930.1983.
Abstract
Mixing suspensions of rabbit antibody for staphylococcal enterotoxin A and commercial anti-rabbit immunoglobulin G-alkaline phosphatase conjugate produced a complex conjugate suitable for use as the second antibody in a conventional enzyme-linked immunosorbent assay. The method was useful for detecting 1 ng of staphylococcal enterotoxin A per ml.
摘要
将兔抗葡萄球菌肠毒素A抗体悬浮液与市售抗兔免疫球蛋白G-碱性磷酸酶结合物混合，产生了一种适合用作传统酶联免疫吸附测定中第二抗体的复合结合物。该方法可用于检测每毫升1纳克的葡萄球菌肠毒素A。
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