用于检测葡萄球菌肠毒素A的双抗体固相酶免疫测定法。
Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.
作者信息
Saunders G C, Bartlett M L
出版信息
Appl Environ Microbiol. 1977 Nov;34(5):518-22. doi: 10.1128/aem.34.5.518-522.1977.
Abstract
A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.
摘要
开发了一种使用微技术的简单双抗体酶免疫测定法，用于检测食品中的葡萄球菌肠毒素A。样品制备可在不到15分钟内完成。测定灵敏度范围为每毫升制备样品中0.4纳克（检测时间20小时）至3.2纳克（检测时间1至3小时）的毒素。从加标食品中分离和检测肠毒素的量占添加量的72%至98%。
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