酶消化后小组织样本中胺碘酮和去乙基胺碘酮的高效液相色谱测定
High-performance liquid chromatographic measurement of amiodarone and desethylamiodarone in small tissue samples after enzymatic digestion.
作者信息
Storey G C, Adams P C, Campbell R W, Holt D W
出版信息
J Clin Pathol. 1983 Jul;36(7):785-9. doi: 10.1136/jcp.36.7.785.
Abstract
A method is described for the measurement of amiodarone and desethylamiodarone in small tissue samples. With the exception of fat, for which lipase is used, the tissues are digested with a proteolytic enzyme. After the addition of an internal standard the analytes are extracted from the homogeneous digest into an organic solvent and measured by high-performance liquid chromatography (HPLC) with UV detection at 240 nm. The method shows good reproducibility using tissue samples as small as 20 mg and suggests extensive accumulation of both compounds in some tissues, with particularly high concentrations in tissues associated with adverse effects of the drug.
摘要
本文描述了一种用于测量小组织样本中胺碘酮和去乙基胺碘酮的方法。除了使用脂肪酶处理的脂肪组织外，其他组织均用蛋白水解酶进行消化。加入内标后，将分析物从均匀的消化液中萃取到有机溶剂中，并通过高效液相色谱法（HPLC）在240nm处进行紫外检测来测定。该方法使用低至20mg的组织样本时显示出良好的重现性，并表明这两种化合物在某些组织中大量蓄积，在与该药物不良反应相关的组织中浓度尤其高。
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本文引用的文献
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2
Side effects of long-term amiodarone therapy.长期胺碘酮治疗的副作用。
Circulation. 1983 Jan;67(1):45-51. doi: 10.1161/01.cir.67.1.45.
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J Cardiovasc Pharmacol. 1982 Mar-Apr;4(2):270-5. doi: 10.1097/00005344-198203000-00016.
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