Martin D E, Glick B
Cell Immunol. 1983 Jul 15;79(2):383-8. doi: 10.1016/0008-8749(83)90080-1.
Thymic (T) or bursal (B) lymphocytes from chicks sensitized to Mycobacterium tuberculosis produce an avian lymphocyte inhibitory factor (LyIF). The physiochemical properties of both T and B LyIF were established by ultrafiltration which yielded four fractions with molecular weight ranges of greater than 100,000; 50,000-100,000; 10,000-50,000; and less than 10,000; enzymatic treatment with chymotrypsin and neuraminidase; varying pH; and heat exposure. These studies demonstrated that the maximum activity for both T and B LyIF was within a molecular weight range of 10,000-50,000. Both were sensitive to chymotrypsin and neuraminidase treatment. Both were stable at 56 degrees C for 30 min and resistant to changes in pH from 5 to 9. T-Cell migration was inhibited equally by B or T LyIF, while B-cell migration was inhibited to a lesser extent by T LyIF and B LyIF. Further experiments should establish the reasons for these observed differences in cross-reactivity.
对结核分枝杆菌致敏的雏鸡的胸腺（T）淋巴细胞或法氏囊（B）淋巴细胞会产生一种禽淋巴细胞抑制因子（LyIF）。通过超滤确定了T和B LyIF的理化性质，超滤产生了四个级分，分子量范围分别大于100,000；50,000 - 100,000；10,000 - 50,000；以及小于10,000；用胰凝乳蛋白酶和神经氨酸酶进行酶处理；改变pH值；以及加热。这些研究表明，T和B LyIF的最大活性都在分子量范围10,000 - 50,000内。两者都对胰凝乳蛋白酶和神经氨酸酶处理敏感。两者在56℃下30分钟稳定，并且在pH值从5到9的变化中具有抗性。T细胞迁移受到B或T LyIF同等程度的抑制，而B细胞迁移受到T LyIF和B LyIF的抑制程度较小。进一步的实验应该确定这些观察到的交叉反应性差异的原因。
