Subba Rao D S, Glick B
Adv Exp Med Biol. 1977;88:87-98. doi: 10.1007/978-1-4613-4169-7_8.
In this study the migration and migration inhibition of different lymphoid cell populations from immunized and control birds were evaluated. Bursal, thymic, and splenic cells migrated in a capillary tube with thymic cells forming the characteristic cone of cells within 4 hours of culture. Both thymic and bursal cell populations from birds sensitized by Mycobacterium tuberculosis produced a lymphocyte inhibitory factor (LyIF) when stimulated by specific antigen in vitro. The ability of bursal cells to release LyIF declined subsequent to 10 weeks of age while LyIF was not apparent in thymic lymphocytes until 9 weeks of age and remained high until 27 weeks of age. The active material generated in specifically stimulated cultures from sensitized bursal cells appeared to be antigen dependent whereas thymic cell mediator is antigen independent. The production of LyIF in chickens, while correlating with delayed type hypersensitivity, does not solely reflect T-cell function but B-cell response as well.
在本研究中，对来自免疫禽类和对照禽类的不同淋巴细胞群体的迁移及迁移抑制情况进行了评估。法氏囊、胸腺和脾脏细胞在毛细管中迁移，胸腺细胞在培养4小时内形成特征性的细胞锥。经结核分枝杆菌致敏的禽类的胸腺和法氏囊细胞群体，在体外受到特异性抗原刺激时会产生淋巴细胞抑制因子（LyIF）。法氏囊细胞释放LyIF的能力在10周龄后下降，而胸腺淋巴细胞中的LyIF直到9周龄才显现，并一直保持高水平直至27周龄。致敏法氏囊细胞在特异性刺激培养物中产生的活性物质似乎依赖于抗原，而胸腺细胞介质则不依赖抗原。鸡体内LyIF的产生虽然与迟发型超敏反应相关，但不仅反映T细胞功能，也反映B细胞反应。
