一种改进的DNA连接酶检测方法揭示了酿酒酵母cdc9突变体中的温度敏感活性。
An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.
作者信息
Barker D G, Johnson A L, Johnston L H
出版信息
Mol Gen Genet. 1985;200(3):458-62. doi: 10.1007/BF00425731.
PMID:3900639
Abstract
A sensitive and quantitative assay for DNA ligase has been developed which is suitable for the analysis of crude cell extracts of yeast. The assay is sufficiently sensitive to detect the low levels of DNA ligase activity remaining in cdc9 mutants of Saccharomyces cerevisiae. Indeed, we have been able to show that this residual activity is temperature-sensitive, thus establishing finally that CDC9 is the structural gene for DNA ligase.
摘要
已开发出一种灵敏且定量的DNA连接酶检测方法，适用于酵母粗细胞提取物的分析。该检测方法灵敏度足够高，能够检测出酿酒酵母cdc9突变体中残留的低水平DNA连接酶活性。实际上，我们已经能够证明这种残余活性是温度敏感型的，从而最终确定CDC9是DNA连接酶的结构基因。
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