正在被积极转录的染色体区域中的化学和构象变化。
Chemical and conformational changes in chromosome regions being actively transcribed.
作者信息
Pagés M, Alonso C
出版信息
Nucleic Acids Res. 1978 Feb;5(2):549-62. doi: 10.1093/nar/5.2.549.
U.V. microspectrophotometry has been used to calculate quantities of nucleic acids and proteins of complete polytene chromosomal sets and specific regions of these chromosomes. It has been found that in chromosomes the ratio of DNA to proteins is approximately 1:4. This ratio however changes when specific regions are compared. The average ratio of DNA to proteins in a puffed region (2-48B4C5) increases to 1:16 in contrast to 1:6 from the same region but in non puffed state. At the same time the RNA quantity increases by a factor of 2. thermal denaturation profiles of formaldehyde fixed chromosomes show that the Tm of this region in puffed and non puffed state differ by 10 degrees C. Moreover these profiles suggest that a large fraction of histone-bound DNA is destabilized during puffing.
紫外显微分光光度法已被用于计算完整多线染色体组以及这些染色体特定区域的核酸和蛋白质含量。研究发现，在染色体中，DNA与蛋白质的比例约为1:4。然而，当比较特定区域时，这一比例会发生变化。与处于非膨胀状态时同一区域1:6的比例相比，膨胀区域（2 - 48B4C5）中DNA与蛋白质的平均比例增加到1:16。与此同时，RNA含量增加了一倍。甲醛固定染色体的热变性图谱表明，该区域在膨胀和非膨胀状态下的解链温度相差10摄氏度。此外，这些图谱表明，在膨胀过程中，与组蛋白结合的DNA的很大一部分变得不稳定。
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