Budtz-Jørgensen E, Kelstrup J, Poulsen S
Acta Odontol Scand. 1983;41(2):93-8. doi: 10.3109/00016358309162308.
The efficacy of a protease preparation (Alcalase, Novo, Denmark) in preventing accumulation of plaque on the fitting surface of complete upper dentures was examined in a double-blind study. Seventeen denture wearers with clinically healthy oral mucosae participated in the study. The protease was dispensed in dissolvent tablets containing 15, 30, 60, 100, 300, 500, or 1000 mg of the enzyme. Enzyme tablets or placebo tablets were dissolved in 150 ml water at 50 degrees C, and the dentures were soaked once daily for 15 min. Denture soaking was performed with or without brushing. The study consisted of 14 1-week periods. At the start of each period the dentures were cleaned by brushing until they showed no visible plaque after staining. At the end of each period plaque was stained and the dentures were photographed. The photographs were projected, and the percentage area of the fitting surface showing plaque deposits was scored with 10% intervals. Statistical analysis showed that there was a significant reduction of plaque scores with increasing enzyme concentration. The most marked reduction in plaque formation was seen when mechanical cleansing was combined with immersion in a 500-mg enzyme solution. At a concentration of 300 mg (or more) the enzyme immersion was as efficient as mechanical cleansing in preventing denture plaque accumulation.
在一项双盲研究中，检测了一种蛋白酶制剂（丹麦诺维信公司生产的碱性蛋白酶）在防止全口上义齿贴合面菌斑积聚方面的效果。17名口腔黏膜临床健康的义齿佩戴者参与了该研究。蛋白酶被制成含有15、30、60、100、300、500或1000毫克该酶的溶解片。酶片或安慰剂片在50摄氏度下溶于150毫升水中，义齿每天浸泡一次，每次15分钟。浸泡义齿时可选择是否刷牙。该研究包括14个为期1周的阶段。在每个阶段开始时，通过刷牙清洁义齿，直到染色后看不到明显菌斑。在每个阶段结束时，对菌斑进行染色并拍摄义齿照片。将照片投影，以10%的间隔对显示菌斑沉积的贴合面面积百分比进行评分。统计分析表明，随着酶浓度的增加，菌斑评分显著降低。当机械清洁与浸泡在500毫克酶溶液中相结合时，菌斑形成减少最为明显。在300毫克（或更高）浓度下，酶浸泡在防止义齿菌斑积聚方面与机械清洁效果相当。
