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美洲螯龙虾中的胰岛素样肽。I. 胰岛素免疫反应性。

Insulin-like peptides in the lobster Homarus americanus. I. Insulin immunoreactivity.

作者信息

Sanders B

出版信息

Gen Comp Endocrinol. 1983 Jun;50(3):366-73. doi: 10.1016/0016-6480(83)90257-5.

DOI:10.1016/0016-6480(83)90257-5
PMID:6350105
Abstract

Experiments were conducted to determine if insulin-like peptides are present in the lobster Homarus americanus. Peptides were found that bind specifically to bovine insulin antibodies in a modified vertebrate radioimmunoassay. Extracts of whole hepatopancreas, gut, and hemolymph contained insulin immunoreactivity (IRI) concentrations of 67.5, 14.0, and 11.0 ng, respectively, per 700-g lobster. No insulin immunoreactivity was detected in neurosecretory cells of the eyestalk. The highest immunoreactivity was measured in the hepatopancreas, in the same fractions of eluate which contained the highest immunoreactivity when a bovine insulin standard was passed through the same chromatographic column.

摘要

进行了实验以确定龙虾美洲螯龙虾中是否存在胰岛素样肽。在改良的脊椎动物放射免疫测定中发现了能与牛胰岛素抗体特异性结合的肽。每700克龙虾的全肝胰腺、肠道和血淋巴提取物中胰岛素免疫反应性(IRI)浓度分别为67.5、14.0和11.0纳克。在眼柄的神经分泌细胞中未检测到胰岛素免疫反应性。当牛胰岛素标准品通过同一色谱柱时,在肝胰腺中测得的免疫反应性最高,洗脱液的相同组分中也含有最高免疫反应性。

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