Gordon L, Buncke H J, Townsend J J
Plast Reconstr Surg. 1978 Apr;61(4):576-9. doi: 10.1097/00006534-197804000-00014.
In both replantation and transplantation, circulation is restored to ischemic muscle by vascular anastomoses. As a result, the length of time that skeletal muscle will endure ischemia is important to know clinically. Using microsurgical techniques, we shut off the blood supply to the quadriceps femoris muscle in adult Wistar rats--by occluding the artery, the vein, or both for varying time intervals. The muscle was maintained in the animal for 48 hours, and then examined histologically. Our results indicate that the period of warm ischemia should be limited to about one hour, because a longer period of arterial ischemia produces severe histological changes in muscle. The limiting factor seems to be the arterial, rather than the venous supply. The possibility of muscle regeneration after such ischemic insults was not investigated.
在再植和移植手术中，通过血管吻合术恢复缺血肌肉的血液循环。因此，了解骨骼肌能够耐受缺血的时长在临床上具有重要意义。我们采用显微外科技术，通过在不同时间段分别阻断成年Wistar大鼠股四头肌的动脉、静脉或动静脉，来切断其血液供应。肌肉在动物体内保留48小时后，进行组织学检查。我们的结果表明，热缺血时间应限制在约1小时，因为较长时间的动脉缺血会导致肌肉出现严重的组织学变化。限制因素似乎是动脉供应，而非静脉供应。本研究未探讨此类缺血损伤后肌肉再生的可能性。
