Muramatsu I
Nihon Seikeigeka Gakkai Zasshi. 1981 Jul;55(7):713-22.
The present study was undertaken to investigate the sequential metabolic and histological changes occurring in dog leg muscles as a consequence of ischemia. The left hind limbs of 45 dogs were amputated at the middle of their thighs and after preserving them in ice water and room temperature for 6 and 12 hours, re-circulation was established. Observation was undertaken on 1) activity of muscle enzymes-CPK, GOT-m, and LDH in the venous blood from the re-circulated limbs, and 2) histological changes of muscles through light microscopy and electron microscopy. Leakage of muscle enzymes into the serum was little in the group preserved in ice water for 6 hours, but resulted in a marked increase in the group preserved in room temperature for 6 hours and 12 hours ischemic groups. This remarkable leakage of muscle enzymes seems to reflect the amount of muscle degeneration. Histologically prominent changes of muscles were characterized by cell swelling and separation of sarcoplasma from endomysium through light microscopic study, and by widening of myofibril space, virtual absence of glycogen granules in sarcoplasma, swelling of mitochondria and appearance of intramitochondrial dense body through electron microscopic study. These histological changes of muscles are well correlated with the leakage of muscle enzymes and demonstrated some extent of irreversible changes had developed already, even in the group preserved in ice water for 6 hours.
本研究旨在调查犬腿部肌肉因缺血而发生的一系列代谢和组织学变化。将45只犬的左后肢在大腿中部截肢，在冰水中保存6小时、室温下保存12小时后，恢复血液循环。观察指标包括：1）再灌注肢体静脉血中肌肉酶（肌酸磷酸激酶、谷草转氨酶m型和乳酸脱氢酶）的活性；2）通过光学显微镜和电子显微镜观察肌肉的组织学变化。在冰水中保存6小时的组中，肌肉酶向血清中的渗漏较少，但在室温下保存6小时和12小时的缺血组中，肌肉酶渗漏显著增加。这种显著的肌肉酶渗漏似乎反映了肌肉变性的程度。通过光学显微镜研究，肌肉组织学上的显著变化表现为细胞肿胀以及肌浆与肌内膜分离；通过电子显微镜研究，表现为肌原纤维间隙增宽、肌浆中糖原颗粒几乎消失、线粒体肿胀以及线粒体内出现致密小体。肌肉的这些组织学变化与肌肉酶的渗漏密切相关，并且表明即使在冰水中保存6小时的组中，也已经出现了一定程度的不可逆变化。
