Bubenik G A, Brown G M, Grota L J
J Histochem Cytochem. 1976 Nov;24(11):1173-7. doi: 10.1177/24.11.63506.
Using fluorescence and double antibody techniques, melatonin was localized immunohistologically in the secretory cells of the Harderian gland of mature male rats. The presence and quantity of melatonin in the acinar cells seem to correlate with the amount of porphyrins inside the lumen. The specificity was proven by disappearance of yellow fluorescence after saturation of antibody with melatonin or after use of nonspecific antibody only.
利用荧光和双抗体技术，褪黑素在成熟雄性大鼠哈德氏腺的分泌细胞中进行了免疫组织化学定位。腺泡细胞中褪黑素的存在和数量似乎与管腔内卟啉的量相关。通过用褪黑素饱和抗体后或仅使用非特异性抗体后黄色荧光消失，证明了其特异性。
