Keren D F, Scott P J, McDonald R A, Wiatrak M
Infect Immun. 1983 Oct;42(1):202-7. doi: 10.1128/iai.42.1.202-207.1983.
Most traditional methods of immunization involve parenteral vaccines. Using a chronically isolated ileal loop model as the probe, we examined the effect of a primary parenteral immunization on the local immune response of the intestine. Secretions from isolated ileal loops of rabbits given a primary parenteral immunization with Shigella flexneri without adjuvant showed a small, but definite, specific immunoglobulin A (IgA) response locally, whereas a vigorous serum antigen-specific IgG response was elicited. Also, stronger antigen-specific IgG activity was detected in secretions of parenterally immunized animals than from animals given shigella only by oral immunization. No local IgA or IgG memory response could be elicited by prior parenteral priming with S. flexneri antigens. Lastly, no increase in the primary local IgA response was found in secretions from animals given an oral dosage regimen previously shown to prime for mucosal memory and then challenged with a single parenteral dose of the same antigen. These studies demonstrate that without adjuvant, parenteral doses of S. flexneri antigens elicit both specific serum and local immune responses, but they are not able to prime for a mucosal memory response upon subsequent mucosal challenge. The implications of these findings for programs that use parenteral vaccination to protect against mucosal diseases are discussed.
大多数传统免疫方法涉及注射用疫苗。我们使用慢性隔离回肠袢模型作为探针，研究了初次注射免疫对肠道局部免疫反应的影响。用无佐剂的弗氏志贺菌进行初次注射免疫的家兔，其隔离回肠袢的分泌物显示出局部有小但明确的特异性免疫球蛋白A（IgA）反应，而同时引发了强烈的血清抗原特异性IgG反应。此外，在注射免疫动物的分泌物中检测到的抗原特异性IgG活性比仅通过口服免疫给予志贺菌的动物更强。先前用弗氏志贺菌抗原进行注射预免疫不能引发局部IgA或IgG记忆反应。最后，在先前已证明可引发黏膜记忆然后用单剂量相同抗原进行注射攻击的动物分泌物中，未发现初次局部IgA反应增加。这些研究表明，无佐剂时，注射剂量的弗氏志贺菌抗原可引发特异性血清和局部免疫反应，但在随后的黏膜攻击时它们不能引发黏膜记忆反应。本文讨论了这些发现对使用注射疫苗预防黏膜疾病计划的意义。
