Keren D F, Collins H H, Gemski P, Holt P S, Formal S B
Infect Immun. 1981 Mar;31(3):1193-202. doi: 10.1128/iai.31.3.1193-1202.1981.
One major stumbling block in the development of an effective means to immunize against shigellosis and other enteric diseases has been the lack of a means to assess sequential mucosal immune responses to different potential immunogens. In the present study, we compared the abilities of live invasive organisms, noninvasive organisms, and nonviable antigen preparations of shigella to elicit mucosal immune responses. Whereas previous studies have found that effective immunity was produced best by vaccination with live invasive strains of shigella, in the present study, live noninvasive strains that did not produce any histopathological damage were consistently able to produce local (immunoglobulin A) immune responses as vigorous as those of the invasive strains. Further, acetone-killed shigella antigen was also an effective mucosal immunogen, whereas hot phenol-water-extracted shigella lipopolysaccharide was ineffective, possibly due to the method of preparation. A single oral or parenteral priming was ineffective in enhancing the mucosal immune response when restimulated 1 month later with the same antigen. However, a mucosal memory response was found to be present several months after a triple mucosal stimulation with a locally invasive vaccine strain.
开发一种有效预防志贺氏菌病和其他肠道疾病的免疫方法的一个主要障碍是缺乏一种评估对不同潜在免疫原的连续粘膜免疫反应的方法。在本研究中，我们比较了活的侵袭性生物体、非侵袭性生物体以及志贺氏菌的无活力抗原制剂引发粘膜免疫反应的能力。虽然先前的研究发现，用活的侵袭性志贺氏菌菌株进行疫苗接种能产生最佳的有效免疫力，但在本研究中，不产生任何组织病理学损伤的活的非侵袭性菌株始终能够产生与侵袭性菌株一样强烈的局部（免疫球蛋白A）免疫反应。此外，丙酮灭活的志贺氏菌抗原也是一种有效的粘膜免疫原，而热酚水提取的志贺氏菌脂多糖则无效，这可能是由于制备方法的原因。当1个月后用相同抗原再次刺激时，单次口服或肠胃外引发在增强粘膜免疫反应方面无效。然而，在用局部侵袭性疫苗菌株进行三次粘膜刺激数月后发现存在粘膜记忆反应。
