Pierce N F, Koster F T
J Immunol. 1980 Jan;124(1):307-11.
Parenteral immunization of rats with cholera toxoid had both priming and suppressive effects upon the antitoxin response in jejunal lamina propria to locally applied toxoid/toxin. Priming was detected when parenteral toxoid was given i.p. but not i.v. or s.c., was enhanced by Freund's adjuvant, and appeared to reflect enhanced encounter of i.p. antigen with IgA-committed lymphocytes in extra-intestinal mucosa-associated lymphoid tissue. In contrast, suppression followed parenteral toxoid given i.p., i.v., or s.c.; suppression was antigen specific and lasted at least 16 weeks. Parenteral toxoid suppressed both primary and secondary types of mucosal antitoxin responses, ultimately preventing the generation of antitoxin-containing immunoblasts from Peyer's patches. Since suppression followed parenteral immunization by routes that did not provoke mucosal priming, it was, at least in those instances, not simply a regulatory consequence of mucosal priming. These results support the notion that priming and suppression of a specific mucosal immune response are independent effects of parenteral immunization that are probably determined by the distribution of antigen to mucosa-associated and systemic lymphoid tissue, respectively.
用霍乱类毒素对大鼠进行胃肠外免疫，对空肠固有层对局部应用的类毒素/毒素的抗毒素反应既有启动作用又有抑制作用。当胃肠外类毒素经腹腔注射而非静脉注射或皮下注射给药时可检测到启动作用，弗氏佐剂可增强该作用，且这似乎反映了腹腔内抗原与肠外黏膜相关淋巴组织中IgA定向淋巴细胞的接触增加。相比之下，抑制作用则在经腹腔、静脉或皮下注射胃肠外类毒素后出现；抑制作用具有抗原特异性，且至少持续16周。胃肠外类毒素抑制黏膜抗毒素反应的初次和二次类型，最终阻止派尔集合淋巴结中产生含抗毒素的免疫母细胞。由于抑制作用是在通过未引发黏膜启动的途径进行胃肠外免疫后出现的，所以至少在这些情况下，它并非仅仅是黏膜启动的调节结果。这些结果支持这样一种观点，即特异性黏膜免疫反应的启动和抑制是胃肠外免疫的独立效应，可能分别由抗原在黏膜相关淋巴组织和全身淋巴组织中的分布所决定。
