Huszar M, Halkin H, Herczeg E, Bubis J, Geiger B
Hum Pathol. 1983 Nov;14(11):1006-8. doi: 10.1016/s0046-8177(83)80182-8.
Immunofluorescent staining of tissue from a lung tumor detected 12 years after excision of a primary malignant melanoma of the skin was negative for prekeratin and positive for vimentin, indicating that the tumor was not epithelial in origin and excluding carcinoma from the differential diagnosis. Complementary conventional staining with hematoxylin-eosin confirmed the melanocytic origin of the tumor, indicating that it was probably an amelanotic metastasis of the original malignant melanoma. The findings in this case demonstrate the potential usefulness of immunohistochemical microscopic characterization of specific intermediate filament proteins in the diagnosis of otherwise ambiguous cases of amelanotic melanoma.
在皮肤原发性恶性黑色素瘤切除12年后检测的肺部肿瘤组织免疫荧光染色显示，前角蛋白为阴性，波形蛋白为阳性，表明该肿瘤并非起源于上皮组织，在鉴别诊断中可排除癌。苏木精 - 伊红的补充传统染色证实了肿瘤的黑素细胞起源，表明它可能是原始恶性黑色素瘤的无色素转移灶。该病例的发现证明了免疫组织化学对特定中间丝蛋白进行微观特征分析在诊断不明确的无色素性黑色素瘤病例中的潜在用途。
