Ramaekers F C, Puts J J, Moesker O, Kant A, Vooijs G P, Jap P H
J Clin Invest. 1983 Mar;71(3):635-43. doi: 10.1172/jci110810.
Intermediate-sized filaments have been studied in human malignant melanomas and in normal melanocytes by immunofluorescence microscopy with antibodies directed against keratin, vimentin, desmin, neurofilament protein, and glial filament protein. Both human melanotic and amelanotic tumor cells and tumor metastases as well as normal melanocytes in human skin and in the rat eye contain exclusively intermediate filaments of the vimentin type. No reaction was seen with antibodies to keratin, desmin, neurofilaments, or glial filaments. These latter four antisera, however, gave strong reactions in epidermis and other epithelial tissues, muscle, or neural tissues, respectively. The results favor a mesenchymal character of melanocytes, although a neuroectodermal origin in an early developmental stage is possible. The finding that melanomas contain exclusively vimentin intermediate filaments may prove useful in differential diagnosis of melanomas from other tumor types.
通过使用针对角蛋白、波形蛋白、结蛋白、神经丝蛋白和神经胶质丝蛋白的抗体进行免疫荧光显微镜检查，对人类恶性黑色素瘤和正常黑素细胞中的中间丝进行了研究。人类有色素性和无色素性肿瘤细胞、肿瘤转移灶以及人类皮肤和大鼠眼中的正常黑素细胞均仅含有波形蛋白类型的中间丝。对角蛋白、结蛋白、神经丝或神经胶质丝的抗体均未出现反应。然而，后四种抗血清分别在表皮和其他上皮组织、肌肉或神经组织中产生了强烈反应。尽管黑素细胞在发育早期可能起源于神经外胚层，但这些结果支持黑素细胞具有间充质特征。黑色素瘤仅含有波形蛋白中间丝这一发现可能在黑色素瘤与其他肿瘤类型的鉴别诊断中有用。
