用于快速区分奈瑟菌属和嗜血杆菌属的检测系统的开发
Development of a test system for rapid differentiation of Neisseria and Haemophilus spp.
作者信息
Eriquez L A, Hodinka N E
出版信息
J Clin Microbiol. 1983 Nov;18(5):1032-9. doi: 10.1128/jcm.18.5.1032-1039.1983.
A qualitative micromethod (IDS Rapid NH system) employing conventional and single-substrate enzyme tests was developed for the biochemical characterization of Neisseria spp., Haemophilus spp., and other gram-negative species. A total of over 140 dehydrated, miniaturized biochemical tests were investigated for their ability to distinguish species. Computer-assisted test selection and pair separation analysis of the data allowed the selection of 11 4-h tests that would identify Haemophilus and Neisseria spp. implicated as etiological agents as well as differentiate them from other Neisseria spp., Moraxella spp., Branhamella catarrhalis, Centers for Disease Control M groups, and Kingella spp. The final test configuration included modified glucose, sucrose, galactosidase, nitrate, phosphatase, resazurin reduction, and two arylamidase tests. In addition, indole, urea, and ornithine decarboxylase tests were included to biochemically type strains of Haemophilus influenzae and Haemophilus parainfluenzae.
开发了一种采用常规和单底物酶试验的定性微量方法（IDS快速NH系统），用于淋病奈瑟菌属、嗜血杆菌属及其他革兰氏阴性菌的生化鉴定。共研究了140多种脱水的小型化生化试验区分菌种的能力。通过计算机辅助的试验选择和数据配对分离分析，选出了11项4小时的试验，这些试验可鉴定作为病原体的嗜血杆菌属和淋病奈瑟菌属，并将它们与其他淋病奈瑟菌属、莫拉克斯氏菌属、卡他布兰汉菌、疾病控制中心M群及金氏菌属区分开来。最终的试验组合包括改良葡萄糖、蔗糖、半乳糖苷酶、硝酸盐、磷酸酶、刃天青还原试验以及两项芳基酰胺酶试验。此外，还包括吲哚、尿素和鸟氨酸脱羧酶试验，用于对流感嗜血杆菌和副流感嗜血杆菌菌株进行生化分型。
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