Cubie H, Edmond E
J Clin Pathol. 1985 Feb;38(2):203-7. doi: 10.1136/jcp.38.2.203.
Five tests for the detection of rubella specific IgM antibody were compared. They were the conventional method of sucrose density gradient fractionation, followed by haemagglutination inhibition; an anti-mu capture radioimmunoassay; and three commercially available enzyme linked assays: Rubazyme M, Rubenz M I, and its successor, Rubenz M II. The five methods detected similar numbers of rubella positive samples between seven and 35 days after the onset of symptoms; in the earlier stages, however, the radioimmunoassay and Rubenz M II were more sensitive. All three commercial kits were straightforward to use but produced misleading positive results with sera containing heterophil antibody. In considering sensitivity, specificity, and cost effectiveness together the Rubenz M tests were the most appropriate for routine use. With the recent withdrawal of Rubenz M I from the market only Rubenz M II is now available. If Epstein-Barr virus infection is excluded, Rubenz M II provides a reliable test for the diagnostic laboratory.
对五种检测风疹特异性IgM抗体的方法进行了比较。它们分别是传统的蔗糖密度梯度分级分离法，随后进行血凝抑制试验；抗μ捕获放射免疫分析法；以及三种市售酶联免疫分析法：Rubazyme M、Rubenz M I及其后续产品Rubenz M II。这五种方法在症状出现后7至35天内检测到的风疹阳性样本数量相似；然而，在早期阶段，放射免疫分析法和Rubenz M II更为敏感。所有三种商用试剂盒使用简便，但对含有嗜异性抗体的血清会产生误导性的阳性结果。综合考虑敏感性、特异性和成本效益，Rubenz M检测方法最适合常规使用。随着Rubenz M I最近退出市场，现在只有Rubenz M II可供使用。如果排除了EB病毒感染，Rubenz M II为诊断实验室提供了一种可靠的检测方法。
