Jeffes E W, Yamamoto R S, Ahmed A R, Granger G A
J Clin Immunol. 1984 Jan;4(1):31-5. doi: 10.1007/BF00915284.
The role of lymphocytes in the pathogenesis of bullous pemphigoid was examined by assaying the blister fluid obtained from bullous pemphigoid patients for the presence of the lymphokine, lymphotoxin. Blister fluids from six bullous pemphigoid were assayed on L-929 target cells for the presence of cytolytic molecules in the standard lymphotoxin assay. Three of six blister fluids obtained from bullous pemphigoid patients and one linear IgA bullous dermatosis patient contained significant levels of cytolytic activity. Control blister fluids from suction blisters, herpes, pemphigus, and toxic epidermal necrolysis patients did not contain cytolytic activity. Serum from five bullous pemphigoid patients also had no cytolytic activity. Neutralization studies using rabbit anti-alpha-lymphotoxin demonstrated that 54 to 88% of the cytolytic activity found in bullous pemphigoid blister fluid was due to alpha-lymphotoxin. These results indicate that lymphotoxin is locally released in the skin of bullous pemphigoid lesions and is detectable in blister fluids.
通过检测大疱性类天疱疮患者水疱液中是否存在淋巴因子淋巴毒素，研究淋巴细胞在大疱性类天疱疮发病机制中的作用。在标准淋巴毒素检测中，对6例大疱性类天疱疮患者的水疱液进行检测，以确定L-929靶细胞上是否存在溶细胞分子。6例大疱性类天疱疮患者和1例线状IgA大疱性皮肤病患者的水疱液中，有3例含有显著水平的溶细胞活性。来自抽吸水疱、疱疹、天疱疮和中毒性表皮坏死松解症患者的对照水疱液不含溶细胞活性。5例大疱性类天疱疮患者的血清也无溶细胞活性。使用兔抗α-淋巴毒素的中和研究表明，大疱性类天疱疮水疱液中54%至88%的溶细胞活性归因于α-淋巴毒素。这些结果表明，淋巴毒素在大疱性类天疱疮皮损皮肤中局部释放，且可在水疱液中检测到。
