Kulp S K, Rikihisa Y, Lin Y C, Moh P P, Li P K, Gu Y
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Parasitol Res. 1993;79(4):279-85. doi: 10.1007/BF00932182.
Gossypol, a natural polyphenolic compound, induces growth-inhibitory and antiparasitic effects in Taenia taeniaeformis metacestodes in vivo and in vitro. We investigated the uptake and localization of [3H]-gossypol in this parasite. Metacestodes were incubated in 10(-5) M [3H]-gossypol at 37 degrees C. Parasites steadily took up tritium activity over the first 3 h of incubation, after which a plateau was maintained for the duration of the experiment. Tissue: medium radioactivity ratios revealed that intralarval tritium activity matched extralarval activity within 30 min of incubation and continued to increase with time. Reverse-phase high-performance liquid chromatographic (HPLC) analysis confirmed tissue incorporation of tritium activity that manifested as a single radioactive species. Autoradiography localized [3H]-gossypol to the tegument, calcareous corpuscles, and parenchyma over the first 2 h of incubation. By 6 h, parenchymal radioactivity had disappeared. T. taeniaeformis metacestodes rapidly take up and accumulate [3H]-gossypol in vitro. This accumulation is apparently selective for specific sites, which may have implications for gossypol's metacestocidal action.
棉酚是一种天然多酚化合物，在体内和体外对绦虫的中绦期幼虫均具有生长抑制和抗寄生虫作用。我们研究了[3H] - 棉酚在这种寄生虫中的摄取和定位情况。将中绦期幼虫在37℃下于10(-5)M [3H] - 棉酚中孵育。在孵育的最初3小时内，寄生虫持续摄取氚活性，之后在实验剩余时间内保持稳定水平。组织与培养基的放射性比率显示，孵育30分钟内幼虫体内的氚活性与幼虫体外活性相当，且随时间持续增加。反相高效液相色谱（HPLC）分析证实了氚活性在组织中的掺入，表现为单一放射性物质。放射自显影显示，在孵育的最初2小时内，[3H] - 棉酚定位于皮层、石灰小体和实质组织。到6小时时，实质组织的放射性消失。绦虫的中绦期幼虫在体外能迅速摄取并积累[3H] - 棉酚。这种积累显然对特定部位具有选择性，这可能与棉酚的杀中绦期幼虫作用有关。
