Bohach G A, Hauser A R, Schlievert P M
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Infect Immun. 1988 Jun;56(6):1665-7. doi: 10.1128/iai.56.6.1665-1667.1988.
The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.
编码B型链球菌致热外毒素的结构基因（命名为speB）被克隆到大肠杆菌中，并定位在一个4.5千碱基的BamHI - BglII DNA片段上。从大肠杆菌克隆株中部分纯化得到的B型链球菌致热外毒素与链球菌来源的毒素具有免疫相关性。此外，来自大肠杆菌或化脓性链球菌的毒素具有相似的淋巴细胞促有丝分裂活性和分子量（29,300），并且在通过等电聚焦评估时显示出相当的微异质性。
