Johnson L P, Schlievert P M, Watson D W
Infect Immun. 1980 Apr;28(1):254-7. doi: 10.1128/iai.28.1.254-257.1980.
Production of group A streptococcal pyrogenic exotoxins (SPE) type A and C was transferred from toxigenic streptococcal strains to nontoxigenic strains by lysogeny. Lysogens were tested for SPE with Ouchterlony immunodiffusion on Todd-Hewitt agar plates; toxin diffusing from isolated colonies reacted with specific hyperimmune antisera to SPE. Phage prepared from strains T25(3) (T12gl) and 3GL16, both yielding SPE type A, formed plaques on T25(3) (NONLYSOGENIC) lawns. Over 90% of the colonies picked from the plaque centers yielded A toxin, suggesting SPE type A was transferred by lysogenic conversion. SPE type C formation was transferred to nontoxigenic strains T25(3) and K56 with supernatant fluids from mitomycin C-induced cultures of CS112, producing SPE types B and C. All lysogens tested were positive for SPE type C, indicating that C toxin induction also was transferred by lysogenic conversion. SPE type B formation was not transferable by lysogeny with the strains tested.
通过溶原性，A 组链球菌致热外毒素（SPE）A 型和 C 型的产生从产毒链球菌菌株转移至无毒菌株。在 Todd-Hewitt 琼脂平板上，用双向免疫扩散法对溶原菌进行 SPE 检测；从分离菌落扩散出的毒素与针对 SPE 的特异性超免疫抗血清发生反应。从均产生 A 型 SPE 的 T25(3)（T12gl）和 3GL16 菌株制备的噬菌体，在 T25(3)（非溶原性）菌苔上形成噬菌斑。从噬菌斑中心挑取的菌落中，超过 90%产生 A 毒素，这表明 A 型 SPE 通过溶原性转变进行转移。用来自丝裂霉素 C 诱导的 CS112 培养物的上清液，将 C 型 SPE 的形成转移至无毒菌株 T25(3)和 K56，CS112 产生 B 型和 C 型 SPE。所有检测的溶原菌对 C 型 SPE 均呈阳性，表明 C 毒素诱导也通过溶原性转变进行转移。在所检测的菌株中，B 型 SPE 的形成不能通过溶原性进行转移。
