Peterson T A, Yochem J, Byers B, Nunn M F, Duesberg P H, Doolittle R F, Reed S I
Nature. 1984;309(5968):556-8. doi: 10.1038/309556a0.
We report here significant primary sequence homology among the predicted translational products of three genes: CDC4 , CDC36 and ets. CDC4 and CDC36 are Saccharomyces cerevisiae cell division cycle genes, while ets is a transformation-specific sequence of avian erythroblastosis virus E26. The deduced primary structures of the three gene products were compared by computer to a large data base of known and predicted protein sequences. The search revealed 22.0-25.5% identity over regions of 140-206 codons, respectively between the different pairwise combinations. For these particular sequences, these identity scores fall 3.4-4.0 standard deviations above the empirically-determined mean values of fortuitous similarity. S. cerevisiae calls require CDC36 and CDC4 in order to complete two early events in the cell cycle: execution of start ( CDC36 ) and spindle pole body separation ( CDC4 ). In virus E26, the ets sequence is linked in frame with delta gag and mybE in the tripartite structure 5'-delta gag- mybE -ets-3', comprising the E26 transforming oncogene. The homologies described here suggest that the biochemical functions or regulation of the CDC4 , CDC36 and ets products may be related.
CDC4、CDC36和ets。CDC4和CDC36是酿酒酵母细胞分裂周期基因，而ets是禽成红细胞增多症病毒E26的转化特异性序列。通过计算机将这三种基因产物的推导一级结构与一个包含已知和预测蛋白质序列的大型数据库进行比较。搜索结果显示，在不同的两两组合之间，140 - 206个密码子区域的同一性分别为22.0% - 25.5%。对于这些特定序列，这些同一性得分比偶然相似性的经验确定平均值高出3.4 - 4.0个标准差。酿酒酵母细胞完成细胞周期中的两个早期事件需要CDC36和CDC4：起始点的执行（CDC36）和纺锤体极体分离（CDC4）。在病毒E26中，ets序列在三方结构5'-δgag - mybE -ets-3'中与δgag和mybE读框相连，该结构包含E26转化致癌基因。此处描述的同源性表明，CDC4、CDC36和ets产物的生化功能或调控可能相关。
