Munson L M, Mandecki W, Caruthers M H, Reznikoff W S
Nucleic Acids Res. 1984 May 11;12(9):4011-7. doi: 10.1093/nar/12.9.4011.
Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.
合成寡核苷酸被用于在lacPUV5启动子中引入突变。相对于转录起始位点,在 -13、-14和 -15位置获得了四个突变。在体内测量了这些突变的影响,并根据该区域的共有序列和其他启动子突变对结果进行了讨论。