Helmerhorst F M, ten Berge M L, van der Plas-van Dalen C M, Engelfriet C P, von dem Borne A E
Vox Sang. 1984;46(5):318-22. doi: 10.1111/j.1423-0410.1984.tb00092.x.
We compared the reactivity of fresh platelets with platelets frozen in dimethylsulfoxide (DMSO) or without a cryopreservative by testing agglutinating and non-agglutinating platelet-specific alloantibodies in titration. Frozen platelets, irrespective of the method of preservation, showed no loss of antigenicity as compared with fresh platelets. The yield of platelets frozen without a cryopreservative proved to be higher (80%) than platelets frozen in the presence of DMSO (50%). Platelets sensitized with autoantibodies from patients with autoimmune thrombocytopenia could also be stored by simple freezing in platelet-rich plasma and used for (re)testing.
我们通过滴定检测凝集性和非凝集性血小板特异性同种抗体，比较了新鲜血小板与在二甲基亚砜（DMSO）中冷冻或未使用冷冻保护剂冷冻的血小板的反应性。与新鲜血小板相比，冷冻血小板无论采用何种保存方法，均未显示出抗原性丧失。事实证明，未使用冷冻保护剂冷冻的血小板回收率（80%）高于在DMSO存在下冷冻的血小板（50%）。来自自身免疫性血小板减少症患者的自身抗体致敏的血小板也可通过简单冷冻于富含血小板的血浆中进行保存，并用于（重新）检测。
