四环素转运至大肠杆菌的能量学
Energetics of tetracycline transport into Escherichia coli.
作者信息
Smith M C, Chopra I
出版信息
Antimicrob Agents Chemother. 1984 Apr;25(4):446-9. doi: 10.1128/AAC.25.4.446.
Abstract
The nature of energy coupling for the active transport of tetracycline into Escherichia coli was examined under conditions in which antibiotic uptake was directly compared with transport of proline (proton motive force dependent) and glutamine (phosphate bond dependent). Tetracycline transport was partially inhibited by osmotic shock and by exposure of bacteria to arsenate, two procedures which substantially reduced glutamine transport. Tetracycline transport was also partially inhibited in an uncB mutant (AN283) exposed to the uncoupler carbonyl cyanide-m-chlorophenyl hydrazone (CCCP) under conditions that inhibited proline transport. Taken together, these data indicate involvement of both phosphate bond hydrolysis and the proton motive force for the active transport of tetracycline into E. coli.
摘要
在将四环素主动转运到大肠杆菌的过程中，对能量偶联的性质进行了研究。研究条件是将抗生素的摄取与脯氨酸（依赖质子动力）和谷氨酰胺（依赖磷酸键）的转运直接进行比较。渗透休克以及将细菌暴露于砷酸盐（这两种操作会大幅降低谷氨酰胺的转运）会部分抑制四环素的转运。在抑制脯氨酸转运的条件下，将uncB突变体（AN283）暴露于解偶联剂羰基氰化物间氯苯腙（CCCP）时，四环素的转运也会受到部分抑制。综合这些数据表明，磷酸键水解和质子动力均参与了四环素向大肠杆菌的主动转运。
相似文献
1
Energetics of tetracycline transport into Escherichia coli.四环素转运至大肠杆菌的能量学
Antimicrob Agents Chemother. 1984 Apr;25(4):446-9. doi: 10.1128/AAC.25.4.446.
2
Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli.敏感型大肠杆菌的膜囊泡对四环素的主动摄取。
Antimicrob Agents Chemother. 1981 Sep;20(3):307-13. doi: 10.1128/AAC.20.3.307.
3
Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli.大肠杆菌中脯氨酸和谷氨酰胺主动运输的不同能量偶联机制。
Proc Natl Acad Sci U S A. 1973 May;70(5):1514-8. doi: 10.1073/pnas.70.5.1514.
4
Uptake of ferrienterochelin by Escherichia coli: energy dependent stage of uptake.大肠杆菌对铁肠螯合素的摄取：摄取的能量依赖阶段。
J Bacteriol. 1977 Apr;130(1):26-36. doi: 10.1128/jb.130.1.26-36.1977.
5
Energy supply for active transport in anaerobically grown Escherichia coli.厌氧生长的大肠杆菌中主动运输的能量供应。
J Bacteriol. 1978 Dec;136(3):844-53. doi: 10.1128/jb.136.3.844-853.1978.
6
Tobramycin uptake in Escherichia coli is driven by either electrical potential or ATP.大肠杆菌中妥布霉素的摄取是由电势或三磷酸腺苷驱动的。
J Bacteriol. 1991 May;173(9):2800-8. doi: 10.1128/jb.173.9.2800-2808.1991.
7
Energization of glucose transport by Pseudomonas fluorescens.荧光假单胞菌对葡萄糖转运的激发作用。
J Bacteriol. 1980 Jun;142(3):755-62. doi: 10.1128/jb.142.3.755-762.1980.
8
Energetics of galactose, proline, and glutamine transport in a cytochrome-deficient mutant of Salmonella typhimurium.鼠伤寒沙门氏菌细胞色素缺陷型突变体中半乳糖、脯氨酸和谷氨酰胺转运的能量学
J Supramol Struct. 1977;6(3):389-98. doi: 10.1002/jss.400060312.
9
Experimental Evolution of Escherichia coli K-12 in the Presence of Proton Motive Force (PMF) Uncoupler Carbonyl Cyanide -Chlorophenylhydrazone Selects for Mutations Affecting PMF-Driven Drug Efflux Pumps.质子动力势（PMF）解偶联剂羰基氰化物-对氯苯腙存在下的大肠杆菌 K-12 的实验进化选择了影响 PMF 驱动的药物外排泵的突变。
Appl Environ Microbiol. 2019 Feb 20;85(5). doi: 10.1128/AEM.02792-18. Print 2019 Mar 1.
10
Energy coupling for methionine transport in Escherichia coli.大肠杆菌中甲硫氨酸转运的能量偶联
J Bacteriol. 1975 Sep;123(3):985-91. doi: 10.1128/jb.123.3.985-991.1975.
引用本文的文献
1
Evaluation of agar dilution as an alternative method for antimicrobial susceptibility testing and tentative ECOFF determination for Arcobacter butzleri.评估琼脂稀释法作为检测空肠弯曲杆菌抗菌药物敏感性的替代方法以及初步确定其流行病学临界浓度。
Sci Rep. 2025 Oct 2;15(1):30335. doi: 10.1038/s41598-025-16223-x.
2
The Transporter-Mediated Cellular Uptake and Efflux of Pharmaceutical Drugs and Biotechnology Products: How and Why Phospholipid Bilayer Transport Is Negligible in Real Biomembranes.药物和生物技术产品的转运体介导的细胞摄取和外排：为什么磷脂双层转运在真实生物膜中可以忽略不计。
Molecules. 2021 Sep 16;26(18):5629. doi: 10.3390/molecules26185629.
3
Tetracycline Antibiotics and Resistance.四环素类抗生素与耐药性
Cold Spring Harb Perspect Med. 2016 Apr 1;6(4):a025387. doi: 10.1101/cshperspect.a025387.
4
Penetration of lipophilic agents with multiple protonation sites into bacterial cells: tetracyclines and fluoroquinolones as examples.具有多个质子化位点的亲脂性药物进入细菌细胞的机制：以四环素和氟喹诺酮类药物为例
Antimicrob Agents Chemother. 1993 Jul;37(7):1393-9. doi: 10.1128/AAC.37.7.1393.
5
Tetracycline uptake by susceptible Escherichia coli cells.
Arch Microbiol. 1985 Apr;141(3):260-5. doi: 10.1007/BF00408069.
6
Leakage of periplasmic proteins from Escherichia coli mediated by polymyxin B nonapeptide.多粘菌素B九肽介导的大肠杆菌周质蛋白渗漏
Antimicrob Agents Chemother. 1986 May;29(5):781-8. doi: 10.1128/AAC.29.5.781.
7
Delta pH-dependent accumulation of tetracycline in Escherichia coli.大肠杆菌中四环素的ΔpH依赖性积累
Antimicrob Agents Chemother. 1991 Jan;35(1):53-6. doi: 10.1128/AAC.35.1.53.
8
Lack of evidence for a saturable tetracycline transport system in Staphylococcus aureus.缺乏金黄色葡萄球菌中可饱和四环素转运系统的证据。
Antimicrob Agents Chemother. 1991 Dec;35(12):2643-4. doi: 10.1128/AAC.35.12.2643.
本文引用的文献
1
Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli.敏感型大肠杆菌的膜囊泡对四环素的主动摄取。
Antimicrob Agents Chemother. 1981 Sep;20(3):307-13. doi: 10.1128/AAC.20.3.307.
2
Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli.大肠杆菌中质粒介导的四环素抗性存在多种机制的证据。
J Gen Microbiol. 1980 Nov;121(1):221-9. doi: 10.1099/00221287-121-1-221.
3
Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic.大肠杆菌中质粒介导的四环素耐药性涉及抗生素外排增加。
Biochem Biophys Res Commun. 1980 Mar 13;93(1):74-81. doi: 10.1016/s0006-291x(80)80247-6.
4
Limitations of a fluorescence assay for studies on tetracycline transport into Escherichia coli.用于研究四环素转运到大肠杆菌中的荧光测定法的局限性。
Antimicrob Agents Chemother. 1983 Jan;23(1):175-8. doi: 10.1128/AAC.23.1.175.
5
Energy coupling in secondary active transport.继发性主动转运中的能量偶联
Biochim Biophys Acta. 1980 May 27;604(1):91-126. doi: 10.1016/0005-2736(80)90586-6.
6
Transport of antibiotics into bacteria.
Adv Microb Physiol. 1982;23:183-240. doi: 10.1016/s0065-2911(08)60338-0.
7
Active accumulation of tetracycline by Escherichia coli.大肠杆菌对四环素的主动积累。
Biochem J. 1970 Jan;116(2):287-97. doi: 10.1042/bj1160287.
8
Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli.大肠杆菌中对休克敏感和抗休克的氨基酸通透酶的不同能量偶联机制。
J Biol Chem. 1974 Dec 25;249(24):7747-55.
9
Reconstitution of oxidative phosphorylation and the adenosine triphosphate-dependent transhydrogenase activity by a combination of membrane fractions from unCA- and uncB- mutant strains of Escherichia coli K12.通过大肠杆菌K12的unCA和uncB突变株的膜组分组合来重建氧化磷酸化和三磷酸腺苷依赖性转氢酶活性。
Biochem J. 1973 Aug;134(4):1015-21. doi: 10.1042/bj1341015.
10
Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli.大肠杆菌中脯氨酸和谷氨酰胺主动运输的不同能量偶联机制。
Proc Natl Acad Sci U S A. 1973 May;70(5):1514-8. doi: 10.1073/pnas.70.5.1514.
Zotero 插件