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来自小麦胚芽的色氨酸转运核糖核酸的结构与功能

Structure and function of tryptophan tRNA from wheat germ.

作者信息

Ghosh K, Ghosh H P

出版信息

Nucleic Acids Res. 1984 Jun 25;12(12):4997-5003. doi: 10.1093/nar/12.12.4997.

DOI:10.1093/nar/12.12.4997
PMID:6377237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318894/
Abstract

The coding properties of tRNATrp from yeast and wheat germ were studied. Unlike E. coli tRNATrp or mitochondrial tRNATrp, eukaryotic tRNATrp did not recognize the UGA codon in vitro. The sequence of wheat germ tRNATrp as determined by [32P] post-labelling techniques is: [sequence in text] The interesting features are: (i) Presence of a C11:G24 base pair in contrast to the U11:G24 in E. coli Su- tRNATrp. (ii) The anticodon sequence is -CmCA- compared to -CCA- in E. coli tRNATrp. (iii) Lack of a hypermodified base i6A adjacent to the 3'-end of the anticodon. (iv) Presence of -T psi CG- sequence instead of -psi psi CG- sequence present in mammalian tRNATrp.

摘要

对来自酵母和小麦胚芽的色氨酸转运RNA（tRNATrp）的编码特性进行了研究。与大肠杆菌tRNATrp或线粒体tRNATrp不同，真核生物tRNATrp在体外不识别UGA密码子。通过[32P]后标记技术确定的小麦胚芽tRNATrp序列为：[文本中的序列]。有趣的特征有：（i）存在C11:G24碱基对，而大肠杆菌琥珀抑制tRNATrp中为U11:G24。（ii）反密码子序列为-CmCA-，而大肠杆菌tRNATrp中为-CCA-。（iii）在反密码子3'端相邻处缺乏超修饰碱基i6A。（iv）存在-TψCG-序列，而非哺乳动物tRNATrp中存在的-ψψCG-序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d9/318894/20e27f55b204/nar00330-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d9/318894/20e27f55b204/nar00330-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d9/318894/20e27f55b204/nar00330-0194-a.jpg

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本文引用的文献

1
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Proc Natl Acad Sci U S A. 1980 Jun;77(6):3164-6. doi: 10.1073/pnas.77.6.3164.
2
Novel features in the genetic code and codon reading patterns in Neurospora crassa mitochondria based on sequences of six mitochondrial tRNAs.基于六种线粒体tRNA序列的粗糙脉孢菌线粒体遗传密码和密码子阅读模式的新特征
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Structural requirements for binding of bovine tRNATrp with avian myeloblastosis virus DNA polymerase.
5'和3'侧翼序列元件对植物色氨酸转运RNA基因体内表达作用的分析。
Plant Cell. 1995 Oct;7(10):1723-34. doi: 10.1105/tpc.7.10.1723.
4
Sequence and codon recognition of bean mitochondria and chloroplast tRNAsTrp: evidence for a high degree of homology.菜豆线粒体和叶绿体tRNA色氨酸的序列及密码子识别：高度同源性的证据
Nucleic Acids Res. 1985 Jun 25;13(12):4411-6. doi: 10.1093/nar/13.12.4411.
5
The leaky UGA termination codon of tobacco rattle virus RNA is suppressed by tobacco chloroplast and cytoplasmic tRNAs(Trp) with CmCA anticodon.烟草脆裂病毒RNA中存在泄漏的UGA终止密码子，该密码子可被具有CmCA反密码子的烟草叶绿体和细胞质色氨酸tRNA（Trp）所抑制。
EMBO J. 1992 Nov;11(11):4167-73. doi: 10.1002/j.1460-2075.1992.tb05510.x.
牛源色氨酸转运RNA（tRNATrp）与禽成髓细胞瘤病毒DNA聚合酶结合的结构要求
Nucleic Acids Res. 1980 Jan 11;8(1):57-66. doi: 10.1093/nar/8.1.57.
4
Studies on polynucleotides. LXXXIX. A study of amino acid incorporation in a reticulocyte cell-free protein-synthesizing system with polyribonucleotides with repeating nucleotide sequences used as messengers.多核苷酸研究。八十九。以具有重复核苷酸序列的多核糖核苷酸作为信使，在无细胞网织红细胞蛋白质合成系统中对氨基酸掺入的研究。
J Biol Chem. 1968 Oct 10;243(19):4959-65.
5
Natural read-through at the UGA termination signal of Q-beta coat protein cistron.Qβ外壳蛋白顺反子UGA终止信号处的天然通读。
Nat New Biol. 1971 Sep 15;234(50):206-9. doi: 10.1038/newbio234206a0.
6
Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor.哺乳动物肽链终止。II. 释放因子的密码子特异性和GTP酶活性。
Proc Natl Acad Sci U S A. 1971 Mar;68(3):619-24. doi: 10.1073/pnas.68.3.619.
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Demonstration of the UAA ochre codon in bakers yeast by amino-acid replacements in iso-1-cytochrome c.通过异-1-细胞色素c中的氨基酸替换在面包酵母中证实UAA赭石密码子。
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