哺乳动物肽链终止。II. 释放因子的密码子特异性和GTP酶活性。
Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor.
作者信息
Beaudet A L, Caskey C T
出版信息
Proc Natl Acad Sci U S A. 1971 Mar;68(3):619-24. doi: 10.1073/pnas.68.3.619.
Abstract
In vitro peptide chain termination with release factor preparations from rabbit reticulocytes, guinea pig liver, or Chinese hamster liver is directed with UAAA, UAGA, or UGAA, suggesting that UAA, UAG, and UGA are terminator condons for mammalian cells. Purified release factor from rabbit reticulocytes has ribosomaldependent GTPase activity, which is stimulated by UAAA. GTP hydrolysis appears requisite for in vitro peptide chain termination in mammals.
摘要
利用来自兔网织红细胞、豚鼠肝脏或中国仓鼠肝脏的释放因子制剂进行的体外肽链终止实验，是由UAAA、UAGA或UGAA引导的，这表明UAA、UAG和UGA是哺乳动物细胞的终止密码子。从兔网织红细胞中纯化得到的释放因子具有核糖体依赖性GTP酶活性，该活性受到UAAA的刺激。GTP水解似乎是哺乳动物体外肽链终止所必需的。
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