Matveeva O V, Shabalina S A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Nucleic Acids Res. 1993 Feb 25;21(4):1007-11. doi: 10.1093/nar/21.4.1007.
Intermolecular hybridization experiments show that murine 18S rRNA and 28S rRNA are capable of forming stable hybrid structures with mRNA from genes p53, c-myc and c-mos from the same species. Both 5'-uncoding and coding oncogene p53 mRNA regions contain fragments interacting with rRNA. Computer analysis revealed 18S rRNA fragments complementary to oligonucleotides frequently met in mRNA, which are potential hybridization regions (clinger-fragments). The distribution of clinger-fragments along 18S rRNA sequence is universal at least for one hundred murine mRNA sequences analyzed. Maximal frequencies of oligonucleotides complementary to 18S rRNA clinger-fragments are reliably (2-3 times) higher for mRNA than for intron sequences and randomly generated sequences. The results obtained suggest a possible role of clinger-fragments in translation processes as universal regions of mRNA binding.
分子间杂交实验表明，小鼠18S rRNA和28S rRNA能够与来自同一物种的p53、c-myc和c-mos基因的mRNA形成稳定的杂交结构。癌基因p53 mRNA的5'-非编码区和编码区均含有与rRNA相互作用的片段。计算机分析显示，18S rRNA片段与mRNA中常见的寡核苷酸互补，这些区域是潜在的杂交区域（附着片段）。至少在分析的一百个小鼠mRNA序列中，附着片段沿18S rRNA序列的分布是普遍的。与18S rRNA附着片段互补的寡核苷酸在mRNA中的最大频率比在内含子序列和随机生成序列中可靠地高（2 - 3倍）。所得结果表明，附着片段作为mRNA结合的通用区域，在翻译过程中可能发挥作用。
