Huntley J F, McGorum B, Newlands G F, Miller H R
Immunology. 1984 Nov;53(3):525-35.
The relationship between mucosal mast cells (MMC) in the intestinal lamina propria, and globule leucocytes (GL) and granulated lymphocytes (GIEL) within the epithelium was compared by analysing the constituents of their granules. Glycosaminoglycan, as well as serine esterase, were present in the granules of all three cell types and the antigenic relationship of these serine esterases to MMC-specific protease (RMCPII) was examined. This enzyme was detected by immunocytochemistry within MMC and GL granules, but not in GIEL. The concentration of RMCPII in preparations of epithelial and lamina propria isolates correlated significantly with the numbers of MMC/GL present within these tissues. RMCPII was detected in epithelial isolates, and in preparations of isolated GIEL, only when MMC and GL were present. These studies add further support to the view that MMC and GL are of a common lineage and strongly suggest that GIEL are unrelated to MMC/GL.
通过分析肠固有层黏膜肥大细胞（MMC）、上皮内的球样白细胞（GL）和颗粒淋巴细胞（GIEL）颗粒的成分，比较了它们之间的关系。所有三种细胞类型的颗粒中均存在糖胺聚糖和丝氨酸酯酶，并检测了这些丝氨酸酯酶与MMC特异性蛋白酶（RMCPII）的抗原关系。通过免疫细胞化学在MMC和GL颗粒中检测到了这种酶，但在GIEL中未检测到。上皮和固有层分离物制剂中RMCPII的浓度与这些组织中MMC/GL的数量显著相关。仅当存在MMC和GL时，才在上皮分离物和分离的GIEL制剂中检测到RMCPII。这些研究进一步支持了MMC和GL具有共同谱系的观点，并强烈表明GIEL与MMC/GL无关。
