Tsuda M, Iino T
J Bacteriol. 1983 Feb;153(2):1018-26. doi: 10.1128/jb.153.2.1018-1026.1983.
Complementation in bacteriophage E79 tv-l-mediated transduction and the phenotypic properties of the flagellar genes in Pseudomonas aeruginosa PAO were investigated by using 195 flagellar mutants of this organism. A total of 15 fla. 1 mot, and 2 che cistrons were identified. At least 5 fla cistrons (fla V to flaZ) and one mot cistron resided in one region, and at least 10 fla cistrons (flaA to flaJ) and two che cistrons (cheA and cheB) resided in another. The flaC mutants exhibited cistron-specific leakiness on motility agar plates. The flaE cistron may be the structural gene for the component protein of the flagellar filament. The cheA mutations, which resulted in pleiotropic phenotypes for flagellar formation, motility, and taxis, belonged to the same complementation group as the flaF mutations; that is, we inferred that cheA and flaF are synonymous.
利用铜绿假单胞菌PAO的195个鞭毛突变体，研究了噬菌体E79 tv - l介导转导中的互补作用以及该菌鞭毛基因的表型特性。共鉴定出15个fla、1个mot和2个che顺反子。至少5个fla顺反子（fla V至flaZ）和1个mot顺反子位于一个区域，至少10个fla顺反子（flaA至flaJ）和2个che顺反子（cheA和cheB）位于另一个区域。flaC突变体在运动性琼脂平板上表现出顺反子特异性渗漏。flaE顺反子可能是鞭毛丝组成蛋白的结构基因。导致鞭毛形成、运动性和趋化性多效表型的cheA突变与flaF突变属于同一互补群；也就是说，我们推断cheA和flaF是同义的。
